Detection of an inversion in the Ty-2 region between S-lycopersicum and S-habrochaites by a combination of de novo genome assembly and BAC cloning

doi:10.1007/s00122-015-2561-6

	Detection of an inversion in the Ty-2 region between S-lycopersicum and S-habrochaites by a combination of de novo genome assembly and BAC cloning
	Wolters, Anne-Marie A.1; Caro, Myluska 1; Dong, Shufang 2; Finkers, Richard 1; Gao, Jianchang 2; Visser, Richard G. F.1; Wang, Xiaoxuan 2; Du, Yongchen 2; Bai, Yuling 1
刊名	THEORETICAL AND APPLIED GENETICS
	2015
卷号	128 期号:10 页码:1987-1997
ISSN号	0040-5752
DOI	10.1007/s00122-015-2561-6
通讯作者	Wolters, Anne-Marie A.
英文摘要	A chromosomal inversion associated with the tomato Ty - 2 gene for TYLCV resistance is the cause of severe suppression of recombination in a tomato Ty - 2 introgression line.Among tomato and its wild relatives inversions are often observed, which result in suppression of recombination. Such inversions hamper the transfer of important traits from a related species to the crop by introgression breeding. Suppression of recombination was reported for the TYLCV resistance gene, Ty-2, which has been introgressed in cultivated tomato (Solanum lycopersicum) from the wild relative S. habrochaites accession B6013. Ty-2 was mapped to a 300-kb region on the long arm of chromosome 11. The suppression of recombination in the Ty-2 region could be caused by chromosomal rearrangements in S. habrochaites compared with S. lycopersicum. With the aim of visualizing the genome structure of the Ty-2 region, we compared the draft de novo assembly of S. habrochaites accession LYC4 with the sequence of cultivated tomato ('Heinz'). Furthermore, using populations derived from intraspecific crosses of S. habrochaites accessions, the order of markers in the Ty-2 region was studied. Results showed the presence of an inversion of approximately 200 kb in the Ty-2 region when comparing S. lycopersicum and S. habrochaites. By sequencing a BAC clone from the Ty-2 introgression line, one inversion breakpoint was identified. Finally, the obtained results are discussed with respect to introgression breeding and the importance of a priori de novo sequencing of the species involved.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000361545100007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100875]
专题	蔬菜花卉研究所_茄科研究室
作者单位	1.Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6700 AJ Wageningen, Netherlands 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wolters, Anne-Marie A.,Caro, Myluska,Dong, Shufang,et al. Detection of an inversion in the Ty-2 region between S-lycopersicum and S-habrochaites by a combination of de novo genome assembly and BAC cloning[J]. THEORETICAL AND APPLIED GENETICS,2015,128(10):1987-1997.
APA	Wolters, Anne-Marie A..,Caro, Myluska.,Dong, Shufang.,Finkers, Richard.,Gao, Jianchang.,...&Bai, Yuling.(2015).Detection of an inversion in the Ty-2 region between S-lycopersicum and S-habrochaites by a combination of de novo genome assembly and BAC cloning.THEORETICAL AND APPLIED GENETICS,128(10),1987-1997.
MLA	Wolters, Anne-Marie A.,et al."Detection of an inversion in the Ty-2 region between S-lycopersicum and S-habrochaites by a combination of de novo genome assembly and BAC cloning".THEORETICAL AND APPLIED GENETICS 128.10(2015):1987-1997.