Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features

doi:10.1186/s12870-015-0639-6

	Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features
	Appiano, Michela 1; Catalano, Domenico 2; Martinez, Miguel Santillan 1; Lotti, Concetta 3; Zheng, Zheng 4; Visser, Richard G. F.1; Ricciardi, Luigi 5; Bai, Yuling 1; Pavan, Stefano 5
刊名	BMC PLANT BIOLOGY
	2015
卷号	15 页码:-
关键词	MLO Powdery mildew Angiosperms Evolution Plant breeding
ISSN号	1471-2229
DOI	10.1186/s12870-015-0639-6
通讯作者	Appiano, Michela
英文摘要	Background: Specific members of the plant Mildew Locus O (MLO) protein family act as susceptibility factors towards powdery mildew (PM), a worldwide-spread fungal disease threatening many cultivated species. Previous studies indicated that monocot and dicot MLO susceptibility proteins are phylogenetically divergent.Methods: A bioinformatic approach was followed to study the type of evolution of Angiosperm MLO susceptibility proteins. Transgenic complementation tests were performed for functional analysis.Results: Our results show that monocot and dicot MLO susceptibility proteins evolved class-specific conservation patterns. Many of them appear to be the result of negative selection and thus are likely to provide an adaptive value. We also tested whether different molecular features between monocot and dicot MLO proteins are specifically required by PM fungal species to cause pathogenesis. To this aim, we transformed a tomato mutant impaired for the endogenous SlMLO1 gene, and therefore resistant to the tomato PM species Oidium neolycopersici, with heterologous MLO susceptibility genes from the monocot barley and the dicot pea. In both cases, we observed restoration of PM symptoms. Finally, through histological observations, we demonstrate that both monocot and dicot susceptibility alleles of the MLO genes predispose to penetration of a non-adapted PM fungal species in plant epidermal cells.Conclusions: With this study, we provide insights on the evolution and function of MLO genes involved in the interaction with PM fungi. With respect to breeding research, we show that transgenic complementation assays involving phylogenetically distant plant species can be used for the characterization of novel MLO susceptibility genes. Moreover, we provide an overview of MLO protein molecular features predicted to play a major role in PM susceptibility. These represent ideal targets for future approaches of reverse genetics, addressed to the selection of loss-of-function resistant mutants in cultivated species.
学科主题	Plant Sciences
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000363289900001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100873]
专题	蔬菜花卉研究所_茄科研究室
作者单位	1.Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6708 PB Wageningen, Netherlands 2.Italian Natl Res Council, Inst Biosci & Bioresources, I-70126 Bari, Italy 3.Univ Foggia, Dept Agr Food & Environm Sci, I-71100 Foggia, Italy 4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 5.Univ Bari, Sect Genet & Plant Breeding, Dept Soil Plant & Food Sci, I-70126 Bari, Italy
推荐引用方式 GB/T 7714	Appiano, Michela,Catalano, Domenico,Martinez, Miguel Santillan,et al. Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features[J]. BMC PLANT BIOLOGY,2015,15:-.
APA	Appiano, Michela.,Catalano, Domenico.,Martinez, Miguel Santillan.,Lotti, Concetta.,Zheng, Zheng.,...&Pavan, Stefano.(2015).Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features.BMC PLANT BIOLOGY,15,-.
MLA	Appiano, Michela,et al."Monocot and dicot MLO powdery mildew susceptibility factors are functionally conserved in spite of the evolution of class-specific molecular features".BMC PLANT BIOLOGY 15(2015):-.