Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers

doi:10.3389/fpls.2016.00927

	Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers
	Shu, Jinshuai; Liu, Yumei; Li, Zhansheng; Zhang, Lili; Fang, Zhiyuan; Yang, Limei; Zhuang, Mu; Zhang, Yangyong; Lv, Honghao
刊名	FRONTIERS IN PLANT SCIENCE
	2016
卷号	7 页码:-
关键词	broccoli cytoplasmic male sterility origin mitochondrial markers diversity
ISSN号	1664-462X
DOI	10.3389/fpls.2016.00927
通讯作者	Shu, Jinshuai
英文摘要	Broccoli (Brassica oleracea var. italica) is an important commercial vegetable crop. As part of an efficient pollination system, cytoplasmic male sterility (CMS) has been widely used for broccoli hybrid production. Identifying the original sources of CMS in broccoli accessions has become an important part of broccoli breeding. In this study, the diversity of the CMS sources of 39 broccoli accessions, including 19 CMS lines and 20 hybrids, were analyzed using mitochondria' markers. All CMS accessions contained the ogu orf/38-related DNA fragment and the key genes of nap CMS, pol CMS, and tour CMS were not detected. The 39 CMS accessions were divided into five groups using six orf/38-related and two simple sequence repeat markers. We observed that ogu CMS R3 constituted 79.49% of the CMS sources. CMS6 and CMS26 were differentiated from the other accessions using a specific primer. CMS32 was distinguished from the other accessions based on a 78-nucleotide deletion at the same locus as the orf/38-related sequence. When the coefficient was about 0.90, five CMS accessions (13CMS6, 13CMS23, 13CMS24, 13CMS37, and 13CMS39) exhibiting abnormal floral organs with poor seed setting were grouped together. The polymerase chain reaction amplification profiles for these five accessions differed from those of the other accessions. We identified eight useful molecular markers that can be used to detect CMS types during broccoli breeding. Our data also provide important information relevant to future studies on the possible origins and molecular mechanisms of CMS in broccoli.
学科主题	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000378380800002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100812]
专题	蔬菜花卉研究所_茄科研究室
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Shu, Jinshuai,Liu, Yumei,Li, Zhansheng,et al. Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers[J]. FRONTIERS IN PLANT SCIENCE,2016,7:-.
APA	Shu, Jinshuai.,Liu, Yumei.,Li, Zhansheng.,Zhang, Lili.,Fang, Zhiyuan.,...&Lv, Honghao.(2016).Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers.FRONTIERS IN PLANT SCIENCE,7,-.
MLA	Shu, Jinshuai,et al."Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers".FRONTIERS IN PLANT SCIENCE 7(2016):-.