A naturally occurring InDel variation in BraA.FLC.b(BrFLC2) associated with flowering time variation in Brassica rapa

doi:10.1186/1471-2229-12-151

	A naturally occurring InDel variation in BraA.FLC.b(BrFLC2) associated with flowering time variation in Brassica rapa
	Wu, Jian 1; Wei, Keyun 1; Cheng, Feng 1; Li, Shikai 2; Wang, Qian 2; Zhao, Jianjun 3; Bonnema, Guusje 3; Wang, Xiaowu 1
刊名	BMC PLANT BIOLOGY
	2012
卷号	12 页码:-
ISSN号	1471-2229
DOI	10.1186/1471-2229-12-151
通讯作者	Wu, Jian
英文摘要	Background: Flowering time is an important trait in Brassica rapa crops. FLOWERING LOCUS C (FLC) is a MADS-box transcription factor that acts as a potent repressor of flowering. Expression of FLC is silenced when plants are exposed to low temperature, which activates flowering. There are four copies of FLC in B. rapa. Analyses of different segregating populations have suggested that BraA.FLC.a (BrFLC1) and BraA.FLC.b (BrFLC2) play major roles in controlling flowering time in B. rapa.Results: We analyzed the BrFLC2 sequence in nine B. rapa accessions, and identified a 57-bp insertion/deletion (InDel) across exon 4 and intron 4 resulting in a non-functional allele. In total, three types of transcripts were identified for this mutated BrFLC2 allele. The InDel was used to develop a PCR-based marker, which was used to screen a collection of 159 B. rapa accessions. The deletion genotype was present only in oil-type B. rapa, including ssp. oleifera and ssp. tricolaris, and not in other subspecies. The deletion genotype was significantly correlated with variation in flowering time. In contrast, the reported splicing site variation in BrFLC1, which also leads to a non-functional locus, was detected but not correlated with variation in flowering time in oil-type B. rapa, although it was correlated with variation in flowering time in vegetable-type B. rapa.Conclusions: Our results suggest that the naturally occurring deletion mutation across exon 4 and intron 4 in BrFLC2 gene contributes greatly to variation in flowering time in oil-type B. rapa. The observed different relationship between BrFLC1 or BrFLC2 and flowering time variation indicates that the control of flowering time has evolved separately between oil-type and vegetable-type B. rapa groups.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000311200800001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101089]
专题	蔬菜花卉研究所_生物技术研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Yunnan Acad Agr Sci, Inst Hort Sci, Kunming 650205, Peoples R China 3.Wageningen Univ, Lab Plant Breeding, NL-6708 PB Wageningen, Netherlands
推荐引用方式 GB/T 7714	Wu, Jian,Wei, Keyun,Cheng, Feng,et al. A naturally occurring InDel variation in BraA.FLC.b(BrFLC2) associated with flowering time variation in Brassica rapa[J]. BMC PLANT BIOLOGY,2012,12:-.
APA	Wu, Jian.,Wei, Keyun.,Cheng, Feng.,Li, Shikai.,Wang, Qian.,...&Wang, Xiaowu.(2012).A naturally occurring InDel variation in BraA.FLC.b(BrFLC2) associated with flowering time variation in Brassica rapa.BMC PLANT BIOLOGY,12,-.
MLA	Wu, Jian,et al."A naturally occurring InDel variation in BraA.FLC.b(BrFLC2) associated with flowering time variation in Brassica rapa".BMC PLANT BIOLOGY 12(2012):-.