Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings

doi:10.1007/s00425-014-2115-1

	Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings
	Dong, Chun-Juan; Li, Liang; Shang, Qing-Mao; Liu, Xin-Yan; Zhang, Zhi-Gang
刊名	PLANTA
	2014
卷号	240 期号:4 页码:687-700
关键词	Cucumis sativus Chilling Salicylic acid Hydrogen peroxide Inhibitor
ISSN号	0032-0935
DOI	10.1007/s00425-014-2115-1
通讯作者	Dong, Chun-Juan
英文摘要	Salicylic acid (SA) is an important plant hormone, and its exogenous application can induce tolerance to multiple environmental stresses in plants. In this study, we examine the potential involvement of endogenous SA in response to chilling in cucumber (Cucumis sativus L.) seedlings. A low temperature of 8 A degrees C induces a moderate increase in endogenous SA levels. Chilling stimulates the enzymatic activities and the expression of genes for phenylalanine ammonia-lyase (PAL) and benzoic acid-2-hydroxylase rather than isochorismate synthase. This indicates that the PAL enzymatic pathway contributes to chilling-induced SA production. Cucumber seedlings pretreated with SA biosynthesis inhibitors accumulate less endogenous SA and suffer more from chilling damage. The expression of cold-responsive genes is also repressed by SA inhibitors. The reduction in stress tolerance and in gene expression can be restored by the exogenous application of SA, confirming the critical roles of SA in chilling responses in cucumber seedlings. Furthermore, the inhibition of SA biosynthesis under chilling stress results in a prolonged and enhanced hydrogen peroxide (H2O2) accumulation. The application of exogenous SA and the chemical scavenger of H2O2 reduces the excess H2O2 and alleviates chilling injury. In contrast, the protective effects of SA are negated by foliar spraying with high concentrations of H2O2 and an inhibitor of the antioxidant enzyme. These results suggest that endogenous SA is required in response to chilling stress in cucumber seedlings, by modulating the expression of cold-responsive genes and the precise induction of cellular H2O2 levels.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000342207300003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100953]
专题	蔬菜花卉研究所_栽培与采后技术研究室
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Dong, Chun-Juan,Li, Liang,Shang, Qing-Mao,et al. Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings[J]. PLANTA,2014,240(4):687-700.
APA	Dong, Chun-Juan,Li, Liang,Shang, Qing-Mao,Liu, Xin-Yan,&Zhang, Zhi-Gang.(2014).Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings.PLANTA,240(4),687-700.
MLA	Dong, Chun-Juan,et al."Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings".PLANTA 240.4(2014):687-700.