Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties

doi:10.1016/S2095-3119(15)61315-6

	Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties
	Bai Miao 1; Yang Guo-shun 1; Chen Wen-ting 1; Lin Run-mao 2; Ling Jiang 2; Mao Zhen-chuan 2; Xie Bing-yang 2
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2016
卷号	15 期号:8 页码:1785-1797
关键词	Tomato yellow leaf curl virus virus-induced RNA silencing virus-derived small RNA degradome
ISSN号	2095-3119
DOI	10.1016/S2095-3119(15)61315-6
通讯作者	Bai Miao
英文摘要	Virus-tolerant plant, which allows the accumulation of virus and then generates virus-derived small RNAs (vsRNAs), is a valuable material to reveal the antiviral efficiency of vsRNAs. Here, a comparison of vsRNAs in Tomato yellow leaf curl virus tolerant and in susceptible tomato varieties showed the consistent trend of vsRNAs' distribution on virus genome, which is presented as an obvious characteristic. However, the expression level of vsRNA in tolerant variety is less than that in susceptible variety. Slicing targets of vsRNA-mediated viral transcripts were investigated using parallel analysis of RNA ends, and geminivirus DNA methylation was determined by bisulfite sequencing, which uncovered that not all vsRNAs participated in viral mRNA degradation and DNA methylation. Additionally, by comparing with the expression pattern of vsRNAs, viral DNA and mRNA, we proposed the quantity of vsRNAs is corresponding to the expression level of viral mRNA, while the virus-suppression of vsRNAs is not high-efficient.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000381539700011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105933]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Bai Miao,Yang Guo-shun,Chen Wen-ting,et al. Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(8):1785-1797.
APA	Bai Miao.,Yang Guo-shun.,Chen Wen-ting.,Lin Run-mao.,Ling Jiang.,...&Xie Bing-yang.(2016).Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties.JOURNAL OF INTEGRATIVE AGRICULTURE,15(8),1785-1797.
MLA	Bai Miao,et al."Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in tolerant and susceptible tomato varieties".JOURNAL OF INTEGRATIVE AGRICULTURE 15.8(2016):1785-1797.