MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing

doi:10.1371/journal.pone.0013271

	MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing
	Huang, Qi-Xing 1,2; Cheng, Xin-Yue 1; Mao, Zhen-Chuan 2; Wang, Yun-Sheng 2,3; Zhao, Li-Lin 4; Yan, Xia 1; Ferris, Virginia R.5; Xu, Ru-Mei 1; Xie, Bing-Yan 2
刊名	PLOS ONE
	2010
卷号	5 期号:10 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0013271
通讯作者	Huang, Qi-Xing
英文摘要	Background: MicroRNAs (miRNAs) are considered to be very important in regulating the growth, development, behavior and stress response in animals and plants in post-transcriptional gene regulation. Pinewood nematode, Bursaphelenchus xylophilus, is an important invasive plant parasitic nematode in Asia. To have a comprehensive knowledge about miRNAs of the nematode is necessary for further in-depth study on roles of miRNAs in the ecological adaptation of the invasive species.Methods and Findings: Five small RNA libraries were constructed and sequenced by Illumina/Solexa deep-sequencing technology. A total of 810 miRNA candidates (49 conserved and 761 novel) were predicted by a computational pipeline, of which 57 miRNAs (20 conserved and 37 novel) encoded by 53 miRNA precursors were identified by experimental methods. Ten novel miRNAs were considered to be species-specific miRNAs of B. xylophilus. Comparison of expression profiles of miRNAs in the five small RNA libraries showed that many miRNAs exhibited obviously different expression levels in the third-stage dispersal juvenile and at a cold-stressed status. Most of the miRNAs exhibited obviously down-regulated expression in the dispersal stage. But differences among the three geographic libraries were not prominent. A total of 979 genes were predicted to be targets of these authentic miRNAs. Among them, seven heat shock protein genes were targeted by 14 miRNAs, and six FMRFamide-like neuropeptides genes were targeted by 17 miRNAs. A real-time quantitative polymerase chain reaction was used to quantify the mRNA expression levels of target genes.Conclusions: Basing on the fact that a negative correlation existed between the expression profiles of miRNAs and the mRNA expression profiles of their target genes (hsp, flp) by comparing those of the nematodes at a cold stressed status and a normal status, we suggested that miRNAs might participate in ecological adaptation and behavior regulation of the nematode. This is the first description of miRNAs in plant parasitic nematodes. The results provide a useful resource for further in-depth study on molecular regulation and evolution of miRNAs in plant parasitic nematodes.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000282807300013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101173]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing, Peoples R China 3.Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha, Hunan, Peoples R China 4.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China 5.Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA
推荐引用方式 GB/T 7714	Huang, Qi-Xing,Cheng, Xin-Yue,Mao, Zhen-Chuan,et al. MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing[J]. PLOS ONE,2010,5(10):-.
APA	Huang, Qi-Xing.,Cheng, Xin-Yue.,Mao, Zhen-Chuan.,Wang, Yun-Sheng.,Zhao, Li-Lin.,...&Xie, Bing-Yan.(2010).MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing.PLOS ONE,5(10),-.
MLA	Huang, Qi-Xing,et al."MicroRNA Discovery and Analysis of Pinewood Nematode Bursaphelenchus xylophilus by Deep Sequencing".PLOS ONE 5.10(2010):-.