Molecular mapping of MS-cd1 gene in Chinese kale

CORC > 中国农业科学院 > 蔬菜花卉研究所 > 职能部门

	Molecular mapping of MS-cd1 gene in Chinese kale
	Zhang, Xin-mei 2; Wu, Jian 1; Guo, Ai-guang 2; Zhang, Hui 1; Ma, Yuan 3; Fang, Zhi-yuan 1; Wang, Xiao-wu 1
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2010
卷号	9 期号:29 页码:4550-4555
关键词	Sequence-related amplified polymorphism DNA markers linkage map Chinese kale
ISSN号	1684-5315
通讯作者	Zhang, Xin-mei
英文摘要	A dominant male sterility (DGMS) line 79-399-3 was developed from spontaneous mutation in Brassica oleracea var. capitata and has been widely used in the production of hybrid cultivar in China. In this line, male sterility is controlled by a dominant gene Ms-cd1. In the present study, primary mapping of Ms-cd1 was conducted by screening a segregating population developed by four times backcrossing of B. oleracea var. alboglabra into a male sterile B. oleracea var. italica line harboring Ms-cd1. Bulked segregation analysis (BSA) was performed for 226 BC(4) individuals using SRAPs regarding of male sterility and fertility. Using 800 SRAP primers and 2,340 SRAP combined random primers, a primary map surrounding Ms-cd1 was constructed. Eight markers closely linked to the target gene were identified, among which the closest one on each side to Ms-cd1 was 0.53 and 5.04 cM, respectively. Markers linked closely to the Ms-cd1 gene will enrich resources of molecular marker of Ms-cd1 locus; also serve to lay the foundations for molecular-assisted selection in breeding program, as well as fine mapping and map-based cloning of Ms-cd1 gene.
学科主题	Biotechnology & Applied Microbiology
语种	英语
出版者	ACADEMIC JOURNALS
WOS记录号	WOS:000280349100007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101177]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.NW Sci & Technol Univ Agr & Forestry, Coll Life Sci, Yangling 712100, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xin-mei,Wu, Jian,Guo, Ai-guang,et al. Molecular mapping of MS-cd1 gene in Chinese kale[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(29):4550-4555.
APA	Zhang, Xin-mei.,Wu, Jian.,Guo, Ai-guang.,Zhang, Hui.,Ma, Yuan.,...&Wang, Xiao-wu.(2010).Molecular mapping of MS-cd1 gene in Chinese kale.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(29),4550-4555.
MLA	Zhang, Xin-mei,et al."Molecular mapping of MS-cd1 gene in Chinese kale".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.29(2010):4550-4555.