Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)

doi:10.1007/s00122-012-1972-x

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	Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)
	Qian, Wei 1; Zhang, Shujiang 1; Zhang, Shifan 1; Li, Fei 1; Zhang, Hui 1; Wu, Jian 1; Wang, Xiaowu 1; Walsh, John A.2; Sun, Rifei 1
刊名	THEORETICAL AND APPLIED GENETICS
	2013
卷号	126 期号:1 页码:179-188
ISSN号	0040-5752
DOI	10.1007/s00122-012-1972-x
通讯作者	Qian, Wei
英文摘要	The extreme resistance to Turnip mosaic virus observed in the Chinese cabbage (Brassica rapa) line, BP8407, is monogenic and recessive. Bulked segregant analysis was carried out to identify simple sequence repeat and Indel markers linked to this recessive resistance gene, termed recessive Turnip mosaic virus resistance 02 (retr02). Mapping of PCR-specific Indel markers on 239 individuals of a BP8407 x Ji Zao Chun F-2 population, located this resistance gene to a 0.9-cM interval between two Indel markers (BrID10694 and BrID101309) and in scaffold000060 or scaffold000104 on chromosome A04 of the B. rapa genome. Eleven eukaryotic initiation factor 4E (eIF4E) and 14 eukaryotic initiation factor 4G (eIF4G) genes are predicted in the B. rapa genome. A candidate gene, Bra035393 on scaffold000104, was predicted within the mapped resistance locus. The gene encodes the eIF(iso)4E protein. Bra035393 was sequenced in BP8407 and Ji Zao Chun. A polymorphism (A/G) was found in exon 3 between BP8407 and Ji Zao Chun. This gene was analysed in four resistant and three susceptible lines. A correlation was observed between the amino acid substitution (Gly/Asp) in the eIF(iso)4E protein and resistance/susceptibility. eIF(iso)4E has been shown previously to interact with the TuMV genome-linked protein, VPg.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000313056900016
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101072]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Chinese Cabbage, Beijing 100081, Peoples R China 2.Univ Warwick, Sch Life Sci, Wellesbourne CV35 9EF, Warwick, England
推荐引用方式 GB/T 7714	Qian, Wei,Zhang, Shujiang,Zhang, Shifan,et al. Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)[J]. THEORETICAL AND APPLIED GENETICS,2013,126(1):179-188.
APA	Qian, Wei.,Zhang, Shujiang.,Zhang, Shifan.,Li, Fei.,Zhang, Hui.,...&Sun, Rifei.(2013).Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.).THEORETICAL AND APPLIED GENETICS,126(1),179-188.
MLA	Qian, Wei,et al."Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)".THEORETICAL AND APPLIED GENETICS 126.1(2013):179-188.