Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

doi:10.1016/j.gene.2013.09.091

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	Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)
	Lei, Yanyuan 1; Zhu, Xun 1; Xie, Wen 1; Wu, Qingjun 1; Wang, Shaoli 1; Guo, Zhaojiang 1; Xu, Baoyun 1; Li, Xianchun 2; Zhou, Xuguo 3; Zhang, Youjun 1
刊名	GENE
	2014
卷号	533 期号:1 页码:180-187
关键词	Bt resistance Cry1Ac Plutella xylostella RNA-seq ABC transporters
ISSN号	0378-1119
DOI	10.1016/j.gene.2013.09.091
通讯作者	Lei, Yanyuan
英文摘要	To investigate the response of Plutella xylostella transcriptome in defending against a Bt toxin, high-throughput RNA-sequencing was carried out to examine Cry1Ac-susceptible and -resistant strains. The comparative analysis indentified over 2900 differentially expressed unigenes (DEUs) between these two strains. Gene Ontology analysis placed these unigenes primarily into cell, cell part, organelle, binding, catalytic, cellular process, metabolic process, and response to stimulus categories. Based on pathway analyses, DEUs were enriched in oxidoreductase activity and membrane lipid metabolic processes, and they were also significantly enriched in pathways related to the metabolic and biosynthesis of secondary metabolites. Most of the unigenes involved in the metabolic pathway were up-regulated in resistant strains. Within the ABC transporter pathway, majority of the down-regulated unigenes belong to ABCC2 and ABCC10, respectively, while up-regulated unigenes were mainly categorized as ABCG2. Furthermore, two aminopeptidases, and four cadherins encoding genes were significantly elevated as well. This study provides a transcriptome foundation for the identification and functional characterization of genes involved in the Bt resistance in an agriculturally important insect pest, P. xylostella. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000327562700026
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101006]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China 2.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA 3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
推荐引用方式 GB/T 7714	Lei, Yanyuan,Zhu, Xun,Xie, Wen,et al. Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)[J]. GENE,2014,533(1):180-187.
APA	Lei, Yanyuan.,Zhu, Xun.,Xie, Wen.,Wu, Qingjun.,Wang, Shaoli.,...&Zhang, Youjun.(2014).Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae).GENE,533(1),180-187.
MLA	Lei, Yanyuan,et al."Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)".GENE 533.1(2014):180-187.