Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp pekinensis)

doi:10.1111/pbr.12200

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	Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp pekinensis)
	Xu, Xiaoyong 1,2; Sun, Xilu 1; Zhang, Yu 1; Zhang, Lugang 1; Fang, Zhiyuan 3
刊名	PLANT BREEDING
	2014
卷号	133 期号:5 页码:615-619
关键词	Heading Chinese cabbage cytoplasmic male sterility BrRfp AFLP SSR
ISSN号	0179-9541
DOI	10.1111/pbr.12200
通讯作者	Xu, Xiaoyong
英文摘要	In this study, AFLP and SSR techniques were combined with the bulk segregant analysis (BSA) method to map the restorer gene BrRfp using an F-2-segregating population comprising 258 individuals developed by crossing the polima (pol)-like cytoplasmic male sterility (CMS) line 06J45 and the restorer line 01S325 of heading Chinese cabbage. A survey of 2048 AFLP primer pairs identified 21 polymorphic fragments, approximately half of which exhibited high similarity with the A09 chromosome sequence of Brassica rapa in the Brassica database (BRAD). Based on the genome sequence, three specific AFLP fragments linked with BrRfp were successfully converted into sequence-characterized amplified region (SCAR) markers, named SC1233, SC2673 and SC2141. Subsequently, 178 pairs of SSR primers were redesigned for further screening, with five producing polymorphic amplification patterns. Linkage analysis showed that these markers were distributed along both sides of the BrRfp gene, with two markers, SSR03 and SSR2528, co-segregating with the BrRfp locus in the F-2 population. These results may be valuable for marker-assisted selection and map-based cloning in heading Chinese cabbage.
学科主题	Agronomy ; Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000342800400009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100951]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Area, Yangling 712100, Shaanxi, Peoples R China 2.Shanxi Agr Univ, Coll Hort, Taigu 030801, Shanxi, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiaoyong,Sun, Xilu,Zhang, Yu,et al. Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp pekinensis)[J]. PLANT BREEDING,2014,133(5):615-619.
APA	Xu, Xiaoyong,Sun, Xilu,Zhang, Yu,Zhang, Lugang,&Fang, Zhiyuan.(2014).Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp pekinensis).PLANT BREEDING,133(5),615-619.
MLA	Xu, Xiaoyong,et al."Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp pekinensis)".PLANT BREEDING 133.5(2014):615-619.