MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth

doi:10.1371/journal.pgen.1005124

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	MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth
	Guo, Zhaojiang 1; Kang, Shi 1; Chen, Defeng 1; Wu, Qingjun 1; Wang, Shaoli 1; Xie, Wen 1; Zhu, Xun 1; Baxter, Simon W.2; Zhou, Xuguo 3; Jurat-Fuentes, Juan Luis 4
刊名	PLOS GENETICS
	2015
卷号	11 期号:4 页码:-
ISSN号	1553-7390
DOI	10.1371/journal.pgen.1005124
通讯作者	Guo, Zhaojiang
英文摘要	Insecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led to field-evolved resistance in several lepidopteran pests. Resistance to Bt Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), was previously mapped to a multigenic resistance locus (BtR-1). Here, we assembled the 3.15 Mb BtR-1 locus and found high-level resistance to Cry1Ac and Bt biopesticide in four independent P. xylostella strains were all associated with differential expression of a midgut membrane-bound alkaline phosphatase (ALP) outside this locus and a suite of ATP-binding cassette transporter subfamily C (ABCC) genes inside this locus. The interplay between these resistance genes is controlled by a previously uncharacterized trans-regulatory mechanism via the mitogen-activated protein kinase (MAPK) signaling pathway. Molecular, biochemical, and functional analyses have established ALP as a functional Cry1Ac receptor. Phenotypic association experiments revealed that the recessive Cry1Ac resistance was tightly linked to down-regulation of ALP, ABCC2 and ABCC3, whereas it was not linked to up-regulation of ABCC1. Silencing of ABCC2 and ABCC3 in susceptible larvae reduced their susceptibility to Cry1Ac but did not affect the expression of ALP, whereas suppression of MAP4K4, a constitutively transcriptionally-activated MAPK upstream gene within the BtR-1 locus, led to a transient recovery of gene expression thereby restoring the susceptibility in resistant larvae. These results highlight a crucial role for ALP and ABCC genes in field-evolved resistance to Cry1Ac and reveal a novel trans-regulatory signaling mechanism responsible for modulating the expression of these pivotal genes in P. xylostella.
学科主题	Genetics & Heredity
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000354524200027
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100910]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100193, Peoples R China 2.Univ Adelaide, Sch Biol Sci, Adelaide, SA, Australia 3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA 4.Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37901 USA
推荐引用方式 GB/T 7714	Guo, Zhaojiang,Kang, Shi,Chen, Defeng,et al. MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth[J]. PLOS GENETICS,2015,11(4):-.
APA	Guo, Zhaojiang.,Kang, Shi.,Chen, Defeng.,Wu, Qingjun.,Wang, Shaoli.,...&Zhang, Youjun.(2015).MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth.PLOS GENETICS,11(4),-.
MLA	Guo, Zhaojiang,et al."MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth".PLOS GENETICS 11.4(2015):-.