Comparative dynamics of ethylene production and expression of the ACS and ACO genes in normal-ripening and non-ripening watermelon fruits

doi:10.1007/s11738-016-2248-x

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Comparative dynamics of ethylene production and expression of the ACS and ACO genes in normal-ripening and non-ripening watermelon fruits
	Zhou, Ming 1,2; Guo, Shaogui 2; Zhang, Jie 2; Zhang, Haiying 2; Li, Changbao 2; Tang, Xiaowei 2; Ren, Yi 2; Gong, Guoyi 2; Xu, Yong 2
刊名	ACTA PHYSIOLOGIAE PLANTARUM
	2016
卷号	38 期号:9 页码:-
关键词	Watermelon Ethylene production ACS ACO Non-climacteric Ripening
ISSN号	0137-5881
DOI	10.1007/s11738-016-2248-x
通讯作者	Zhou, Ming
英文摘要	Watermelon fruit is traditionally classified as non-climacteric, but it has been reported to be hypersensitive to exogenous ethylene: resulting in accelerated softening and water-soaking. The previous studies have reported a modest, but significant increase in ethylene production in strawberry, grape, etc., which has stimulated interest in exploring the possible role of this hormone in ripening of non-climacteric fruits. In this study, both ethylene emission and content of internal ethylene (CIE) in watermelon fruit were determined, with tomato fruit used as the reference. Throughout the development and ripening, the average CIE levels in flesh was higher than the nearly undetectable ethylene emission from fruits, and revealed a distinct rise of ethylene production in flesh in normal-ripening line 97103, but not in non-ripening line PI296341-FR. In parallel with the patterns of CIE, the activity of 1-aminocyclopropane-1-carboxylate synthase (ACS) and 1-aminocyclopropane-1-carboxylate oxidase (ACO) both increased in 97103 flesh as ripening progressed, but stayed at a constant and low level in PI296341-FR flesh. Eight ACS and eight ACO isoforms were identified in watermelon genome by full-length alignment and domain analysis of protein sequences, and among which, four ACS and four ACO genes were selected based on transcriptome profiling of the fruit development for further assay using qPCR. The expression of two ACS isoforms, Cla022653 and Cla011522, as well as two ACO isoforms, Cla014827 and Cla016287, were significantly up-regulated in 97103 flesh during ripening compared with PI296341-FR, indicating that these four genes could potentially play a more important role in ethylene biosynthesis in normal-ripening watermelon flesh.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000382676100017
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105906]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Ming,Guo, Shaogui,Zhang, Jie,et al. Comparative dynamics of ethylene production and expression of the ACS and ACO genes in normal-ripening and non-ripening watermelon fruits[J]. ACTA PHYSIOLOGIAE PLANTARUM,2016,38(9):-.
APA	Zhou, Ming.,Guo, Shaogui.,Zhang, Jie.,Zhang, Haiying.,Li, Changbao.,...&Xu, Yong.(2016).Comparative dynamics of ethylene production and expression of the ACS and ACO genes in normal-ripening and non-ripening watermelon fruits.ACTA PHYSIOLOGIAE PLANTARUM,38(9),-.
MLA	Zhou, Ming,et al."Comparative dynamics of ethylene production and expression of the ACS and ACO genes in normal-ripening and non-ripening watermelon fruits".ACTA PHYSIOLOGIAE PLANTARUM 38.9(2016):-.