Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts

doi:10.1016/j.gene.2012.05.041

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts
	Yan, Xia 2; Cheng, Xin-Yue 2; Wang, Yun-Sheng 1; Luo, Ji 2; Mao, Zhen-Chuan 1; Ferris, Virginia R.3; Xie, Bing-Yan 1
刊名	GENE
	2012
卷号	505 期号:1 页码:81-90
关键词	Bursaphelenchus xylophilus Bursaphelenchus mucronatus Parasitism-related gene Venom allergen-like protein Xenobiotic degradation
ISSN号	0378-1119
DOI	10.1016/j.gene.2012.05.041
通讯作者	Yan, Xia
英文摘要	Bursaphelenchus xylophilus and Bursaphelenchus mucronatus are migratory endoparasitic nematodes that live in pine trees. To gain insight into their molecular similarities and differences, transcriptomes of the two nematodes were analysed. A total of 23,765 and 21,782 contigs (>300 bp) were obtained from B. xylophilus and B. mucronatus, respectively. More than 80% of the contigs could map to each other's transcriptome reciprocally. A total of 23,467 and 21,370 Open Reading Frames were predicted, respectively. Besides those known parasitism-related proteins, six new venom allergen-like proteins (VAPs) were found, which were not homologous to known VAPs. Enzymes involved in xenobiotic biodegradation were abundant in the two transcriptomes based on KEGG functional annotation. Metabolism of xenobiotics by cytochrome P450 comprised the main detoxification pathways. The mRNA expression levels of detoxification genes in nematodes living in the host were higher than those in nematodes feeding on fungus. However, there were fewer enzymes involved in the a-pinene degradation. Our results indicate that the two pinewood nematodes have evolved similar molecular mechanisms to adapt to life on pine hosts. (C) 2012 Elsevier B.v. All rights reserved.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000307609100011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101919]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China 3.Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA
推荐引用方式 GB/T 7714	Yan, Xia,Cheng, Xin-Yue,Wang, Yun-Sheng,et al. Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts[J]. GENE,2012,505(1):81-90.
APA	Yan, Xia.,Cheng, Xin-Yue.,Wang, Yun-Sheng.,Luo, Ji.,Mao, Zhen-Chuan.,...&Xie, Bing-Yan.(2012).Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts.GENE,505(1),81-90.
MLA	Yan, Xia,et al."Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts".GENE 505.1(2012):81-90.