A high-resolution cucumber cytogenetic map integrated with the genome assembly

doi:10.1186/1471-2164-14-461

CORC > 中国农业科学院 > 蔬菜花卉研究所

	A high-resolution cucumber cytogenetic map integrated with the genome assembly
	Sun, Jianying 1; Zhang, Zhonghua 2,3; Zong, Xu 1; Huang, Sanwen 2,3; Li, Zongyun 1,4; Han, Yonghua 1,4
刊名	BMC GENOMICS
	2013
卷号	14 页码:-
ISSN号	1471-2164
DOI	10.1186/1471-2164-14-461
通讯作者	Sun, Jianying
英文摘要	Background: High-resolution cytogenetic map can provide not only important biological information on genome organization but also solid foundation for genetic and genomic research. The progress in the molecular and cytogenetic studies has created the basis for developing the cytogenetic map in cucumber (Cucumis sativus L.).Results: Here, the cytogenetic maps of four cucumber chromosomes (chromosomes 1, 3-5) were constructed by fluorescence in situ hybridization (FISH) analysis on cucumber pachytene chromosomes. Together with our previously constructed cytogenetic maps of three cucumber chromosomes (chromosomes 2, 6-7), cucumber has a complete cytogenetic map with 76 anchoring points between the genetic, the cytogenetic and the draft genome assembly maps. To compare our pachytene FISH map directly to the genetic linkage and draft genome assembly maps, we used a standardized map unit-relative map position (RMP) to produce the comparative map alignments. The alignments allowed a global view of the relationship of genetic and physical distances along each cucumber chromosome, and accuracy and coverage of the draft genome assembly map.Conclusions: We demonstrated a good correlation between positions of the markers in the linkage and physical maps, and essentially complete coverage of chromosome arms by the draft genome assembly. Our study not only provides essential information for the improvement of sequence assembly but also offers molecular tools for cucumber genomics research, comparative genomics and evolutionary study.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; GENETICS & HEREDITY
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000321777800001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101887]
专题	蔬菜花卉研究所
作者单位	1.Jiangsu Normal Univ, Inst Integrat Plant Biol, Sch Life Sci, Xuzhou 221116, Peoples R China 2.Chinese Acad Agr Sci, Minist Agr, Key Lab Hort Crops Genet Improvement, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 4.Jiangsu Normal Univ, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jianying,Zhang, Zhonghua,Zong, Xu,et al. A high-resolution cucumber cytogenetic map integrated with the genome assembly[J]. BMC GENOMICS,2013,14:-.
APA	Sun, Jianying,Zhang, Zhonghua,Zong, Xu,Huang, Sanwen,Li, Zongyun,&Han, Yonghua.(2013).A high-resolution cucumber cytogenetic map integrated with the genome assembly.BMC GENOMICS,14,-.
MLA	Sun, Jianying,et al."A high-resolution cucumber cytogenetic map integrated with the genome assembly".BMC GENOMICS 14(2013):-.