Identification of S haplotypes in cabbage inbred lines (Brassica oleracea var. capitata L.)

doi:10.1016/j.scienta.2013.09.042

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Identification of S haplotypes in cabbage inbred lines (Brassica oleracea var. capitata L.)
	Tian, Lei; Miao, Wenwen; Liu, Jisheng; Fang, Zhiyuan; Liu, Yumei; Yang, Limei; Zhang, Yangyong; Zhuang, Mu
刊名	SCIENTIA HORTICULTURAE
	2013
卷号	164 页码:400-408
关键词	Cabbage Self-incompatibility S haplotype SRK SCR Fluorescence microscopy
ISSN号	0304-4238
DOI	10.1016/j.scienta.2013.09.042
通讯作者	Tian, Lei
英文摘要	Knowledge of the S haplotypes of cabbage breeding lines is very important for breeders when assuring cross-compatibility between lines. In the present study, PCR amplification using gene-specific primers derived from the S locus receptor kinase (SRK) and S locus cysteine protein (SCR) genes, followed by sequencing and BLAST analysis, allowed the molecular identification of the S haplotypes of 111 cabbage inbred lines. The identified S haplotypes were confirmed by fluorescence microscopy after pollination in those inbred lines with the same flowering time. Using this method, class I and class II haplotypes were detected in 56 and 55 inbred lines, respectively. Sixteen known S haplotypes were observed in 107 inbred lines; however, four class I lines could not be identified by sequence analysis. Recombination between the sequences encoding the S and kinase domains of the SRK protein was observed in four inbred lines indicating that the sequence of the SRK S domain is more important than that of the SRK kinase domain for identification of S haplotypes. In the study, S15 was the most common haplotype, with a frequency of 21.62%, while S13, S16 and S35 were the lowest frequency haplotypes (0.90%). Most inbred lines with class I haplotypes showed stronger self-incompatibility, and self-compatible inbred lines were mainly from S5 and S15. The distribution of S haplotypes in cabbage inbred lines will be useful for the breeding objectives and may guide future development of new self-compatible inbred lines and F-1 hybrids. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	Horticulture ; HORTICULTURE
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000330200700054
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101861]
专题	蔬菜花卉研究所
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Tian, Lei,Miao, Wenwen,Liu, Jisheng,et al. Identification of S haplotypes in cabbage inbred lines (Brassica oleracea var. capitata L.)[J]. SCIENTIA HORTICULTURAE,2013,164:400-408.
APA	Tian, Lei.,Miao, Wenwen.,Liu, Jisheng.,Fang, Zhiyuan.,Liu, Yumei.,...&Zhuang, Mu.(2013).Identification of S haplotypes in cabbage inbred lines (Brassica oleracea var. capitata L.).SCIENTIA HORTICULTURAE,164,400-408.
MLA	Tian, Lei,et al."Identification of S haplotypes in cabbage inbred lines (Brassica oleracea var. capitata L.)".SCIENTIA HORTICULTURAE 164(2013):400-408.