A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)

doi:10.1371/journal.pone.0148422

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	A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)
	Cui, Jin-Ying 1; Miao, Han 1; Ding, Li-Hong 1; Wehner, Todd C.2; Liu, Pan-Na 1; Wang, Ye 1; Zhang, Sheng-Ping 1; Gu, Xing-Fang 1
刊名	PLOS ONE
	2016
卷号	11 期号:2 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0148422
通讯作者	Cui, Jin-Ying
英文摘要	Spines or trichomes on the fruit of cucumbers enhance their commercial value in China. In addition, glabrous mutants exhibit resistance to aphids and therefore their use by growers can reduce pesticide residues. Previous studies have reported two glabrous mutant plants containing the genes, csgl1 and csgl2. In the present study, a new glabrous mutant, NCG157, was identified showing a gene interaction effect with csgl1 and csgl2. This mutant showed the glabrous character on stems, leaves, tendrils, receptacles and ovaries, and there were no spines or tumors on the fruit surface. Inheritance analysis showed that a single recessive gene, named csgl3, determined the glabrous trait. An F-2 population derived from the cross of two inbred lines 9930 (a fresh market type from Northern China that exhibits trichomes) and NCG157 (an American processing type with glabrous surfaces) was used for genetic mapping of the csgl3 gene. By combining bulked segregant analysis (BAS) with molecular markers, 18 markers, including two simple sequence repeats (SSR), nine insertion deletions (InDel) and seven derived cleaved amplified polymorphism sequences (dCAPs), were identified to link to the csgl3 gene. All of the linked markers were used as anchor loci to locate the csgl3 gene on cucumber chromosome 6. The csgl3 gene was mapped between the dCAPs markers dCAPs-21 and dCAPs-19, at genetic distances of 0.05 cM and 0.15 cM, respectively. The physical distance of this region was 19.6 kb. Three markers, InDel-19, dCAPs-2 and dCAPs-11, co-segregated with csgl3. There were two candidate genes in the region, Csa6M514860 and Csa6M514870. Quantitative real-time PCR showed that the expression of Csa6M514870 was higher in the tissues of 9930 than that of NCG157, and this was consistent with their phenotypic characters. Csa6M514870 is therefore postulated to be the candidate gene for the development of trichomes in cucumber. This study will facilitate marker-assisted selection (MAS) of the smooth plant trait in cucumber breeding and provide for future cloning of csgl3.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000369552800056
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101754]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100193, Peoples R China 2.N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA
推荐引用方式 GB/T 7714	Cui, Jin-Ying,Miao, Han,Ding, Li-Hong,et al. A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)[J]. PLOS ONE,2016,11(2):-.
APA	Cui, Jin-Ying.,Miao, Han.,Ding, Li-Hong.,Wehner, Todd C..,Liu, Pan-Na.,...&Gu, Xing-Fang.(2016).A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.).PLOS ONE,11(2),-.
MLA	Cui, Jin-Ying,et al."A New Glabrous Gene (csgl3) Identified in Trichome Development in Cucumber (Cucumis sativus L.)".PLOS ONE 11.2(2016):-.