A SIX1 Homolog in Fusarium oxysporum f. sp conglutinans Is Required for Full Virulence on Cabbage

doi:10.1371/journal.pone.0152273

CORC > 中国农业科学院 > 蔬菜花卉研究所

	A SIX1 Homolog in Fusarium oxysporum f. sp conglutinans Is Required for Full Virulence on Cabbage
	Li, Erfeng 1; Wang, Gang 1; Xiao, Jiling 2; Ling, Jian 1; Yang, Yuhong 1; Xie, Bingyan 1
刊名	PLOS ONE
	2016
卷号	11 期号:3 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0152273
通讯作者	Li, Erfeng
英文摘要	Fusarium oxysporum is a soil-born fungus that induces wilt and root rot on a variety of plants. F. oxysporum f. sp. conglutinans (Foc) can cause wilt disease on cabbage. This study showed that a homolog of SIX1 protein in the Arabidopsis infecting isolate Fo5176 (Fo5176-SIX1) had four isoforms in the conidia of Foc by proteomic analysis. Thus, we analyzed the roles of protein Foc-SIX1. Gene expression analysis showed that, compared to the expression in mycelia, dramatically altered expression of Foc-SIX1 could be detected after infecting cabbages, and Foc-SIX1 was highly expressed in conidia under axenic culture condition. Furthermore, we knocked out the Foc-SIX1 gene and found that Foc-Delta SIX1 mutants had significantly reduced virulence compared with wild type isolate, and full virulence was restored by complementation of Foc-Delta SIX1 mutants with Foc-SIX1. Thus, we concluded that SIX1 in Foc was required for full virulence on cabbage. We also complemented Foc-Delta SIX1 with SIX1 gene in F. oxysporum f. sp. lycopersici (Fol) and found Foc-Delta SIX1::Fol-SIX1 mutants did not affect the virulence of Foc-Delta SIX1. The results confirmed that Fol-SIX1 was not capable of replacing the role of Foc-SIX1 in Foc on the disease symptom development of cabbage. The roles of Fol-SIX1 on virulence might rely on host specificity.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000372708000125
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101748]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Hunan Watermelon & Muskmelon Inst, Changsha 410125, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Li, Erfeng,Wang, Gang,Xiao, Jiling,et al. A SIX1 Homolog in Fusarium oxysporum f. sp conglutinans Is Required for Full Virulence on Cabbage[J]. PLOS ONE,2016,11(3):-.
APA	Li, Erfeng,Wang, Gang,Xiao, Jiling,Ling, Jian,Yang, Yuhong,&Xie, Bingyan.(2016).A SIX1 Homolog in Fusarium oxysporum f. sp conglutinans Is Required for Full Virulence on Cabbage.PLOS ONE,11(3),-.
MLA	Li, Erfeng,et al."A SIX1 Homolog in Fusarium oxysporum f. sp conglutinans Is Required for Full Virulence on Cabbage".PLOS ONE 11.3(2016):-.