Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L

doi:10.1016/S2095-3119(15)61275-8

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L
	Cao Yacong 1; Zhang Zhenghai 2; Wang Lihao 2; Sui Xiaolei 1; Zhang Zhenxian 1; Zhang Baoxi 2
刊名	Journal of Integrative Agriculture
	2016
卷号	15 期号:4 页码:775-784
关键词	Capsicum annuum L. fruit GID1 DELLA
ISSN号	2095-3119
DOI	10.1016/S2095-3119(15)61275-8
英文摘要	Fruit set and development are affected by many phytohormones, including gibberellin. Little is known regarding molecular mechanism underlying gibberellin mediated fruit set and development especially in Capsicum. Three gibberellin receptors, CaGID1b.1, CaGID1b.2 and CaGID1c, and a DELLA protein, CaGAI, have been identified in Capsicum annuum L. During the fruit development, the expression level of CaGID1c was low, and the expression fold change is mild. However, CaGID1b.1 and CaGID1b.2 were relatively higher and more acute, which indicates that CaGID1b.1 and CaGID1b.2 may play an important role in fruit pericarp, placenta and seed. Ectopic expressions of CaGID1b.1, CaGID1b.2 and CaGID1c in Arabidopsis double mutant gid1a gid1c increased plant height, among which CaGID1b.2 had the most significant effect; CaGAI reduced plant height in double mutant rga-24/gai-t6, having a similar function to AtGID1 and AtGAI in stem elongation. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that CaGID1b.1 and CaGID1b.2 interact with CaGAI in a GA-dependent manner, while CaGID1c interacts with CaGAI in a GA-independent manner. Our study reveals the key elements during gibberellin signaling in Capsicum and supports the critical importance of gibberellin for Capsicum fruit set and development.
学科主题	园艺
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100559]
专题	蔬菜花卉研究所
作者单位	1.China Agricultural University, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops 2.Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, Ministry of Agriculture, Beijing, 100193 3.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, Ministry of Agriculture, Beijing, 100081
推荐引用方式 GB/T 7714	Cao Yacong,Zhang Zhenghai,Wang Lihao,et al. Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L[J]. Journal of Integrative Agriculture,2016,15(4):775-784.
APA	Cao Yacong,Zhang Zhenghai,Wang Lihao,Sui Xiaolei,Zhang Zhenxian,&Zhang Baoxi.(2016).Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L.Journal of Integrative Agriculture,15(4),775-784.
MLA	Cao Yacong,et al."Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L".Journal of Integrative Agriculture 15.4(2016):775-784.