A method to construct a third-generation horseradish peroxidase biosensor: Self-assembling gold nanoparticles to three-dimensional sol-gel network

	A method to construct a third-generation horseradish peroxidase biosensor: Self-assembling gold nanoparticles to three-dimensional sol-gel network
	Jia JB ; Wang BQ ; Wu AG ; Cheng GJ ; Li Z ; Dong SJ
刊名	analytical chemistry
	2002
卷号	74 期号:9 页码:2217-2223
关键词	DIRECT ELECTRON-TRANSFER DIRECT ELECTROCHEMISTRY HYDROGEN-PEROXIDE COLLOIDAL GOLD ENZYME ELECTRODES AMPEROMETRIC BIOSENSORS GRAPHITE ELECTRODE AU COLLOIDS SILICA-GEL MONOLAYERS
ISSN号	0003-2700
通讯作者	dong sj
中文摘要	a novel method for fabrication of horseradish peroxidase biosensor has been developed by self-assembling gold nanoparticles to a thiol-containing sol-gel network. a cleaned gold electrode was first immersed in a hydrolyzed (3-mercaptopropyl)-trimethoxysilane (mps) sol-gel solution to assemble three-dimensional silica gel, and then gold nanoparticles were chemisorbed onto the thiol groups of the sol-gel network. finally, horseradish peroxidase (hrp) was adsorbed onto the surface of the gold nanoparticles. the distribution of gold nanoparticles and hrp was examined by atomic force microscopy (afm). the immobilized horseradish peroxidase exhibited direct electrochemical behavior toward the reduction of hydrogen peroxide. the performance and factors influencing the performance of the resulting biosensor were studied in detail. the resulting biosensor exhibited fast amperometric response (2.5 s) to h2o2. the detection limit of the biosensor was 2.0 mumol l-1, and the linear range was from 5.0 mumol l-1 to 10.0 mmol l-1. moreover, the studied biosensor exhibited high sensitivity, good reproducibility, and long-term stability.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000175358800067
公开日期	2010-11-03
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/18633]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Jia JB,Wang BQ,Wu AG,et al. A method to construct a third-generation horseradish peroxidase biosensor: Self-assembling gold nanoparticles to three-dimensional sol-gel network[J]. analytical chemistry,2002,74(9):2217-2223.
APA	Jia JB,Wang BQ,Wu AG,Cheng GJ,Li Z,&Dong SJ.(2002).A method to construct a third-generation horseradish peroxidase biosensor: Self-assembling gold nanoparticles to three-dimensional sol-gel network.analytical chemistry,74(9),2217-2223.
MLA	Jia JB,et al."A method to construct a third-generation horseradish peroxidase biosensor: Self-assembling gold nanoparticles to three-dimensional sol-gel network".analytical chemistry 74.9(2002):2217-2223.