Layer-by-layer assembly of multilayer films composed of avidin and biotin-labeled antibody for immunosensing

	Layer-by-layer assembly of multilayer films composed of avidin and biotin-labeled antibody for immunosensing
	Cui XQ ; Pei RJ ; Wang XZ ; Yang F ; Ma Y ; Dong SJ ; Yang XR
刊名	biosensors & bioelectronics
	2003
卷号	18 期号:1 页码:59-67
关键词	BIOSPECIFIC INTERACTION ANALYSIS PLASMON RESONANCE SENSORS THIN-FILMS POLYELECTROLYTE FILMS GLUCOSE-OXIDASE 3-DIMENSIONAL STRUCTURE PLATINUM-ELECTRODE GOLD SURFACES PROTEIN CONSTRUCTION
ISSN号	0956-5663
通讯作者	yang xr
中文摘要	protein multilayers composed of avidin and biotin-labeled antibody (bio-ab) were prepared on gold surface by layer-by-layer assembly technology using the high specific binding constant (k-a: approximate to 10(15) m-1) between avidin and biotin. the assembly process of the multilayer films was monitored by using real-time bia technique based on surface plasmon resonance (spr). the multilayer films were also characterized by electrochemical impedance spectroscopy (eis) and reflection absorption fourier transform infrared spectroscopy (ftir). the results indicate that the growth of the multilayer is uniform. from response of spr for each layer, the stoichiometry s for the interaction between avidin and bio-ab is calculated to be 0.37 in the multilayer whereas 0.82 in the first layer. the protein mass concentration for each layer was also obtained. the schematic figure for the multilayer assembly was proposed according to the layer mass, concentration and s value. the utility of the mutilayer films for immunosensing has been investigated via their subsequent interaction with higg. the binding ability of the multilayer increased for one to three layers of antibody, and then reach saturation after the fourth layer. these layer-by-layer constructed antibody multilayers enhance the binding ability than covalently immobilized monolayer antibody. this technology can be also used for construction of other thin films for immunosensing and biosensor.
收录类别	SCI
语种	英语
WOS记录号	WOS:000180412700007
公开日期	2010-09-13
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/17887]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Cui XQ,Pei RJ,Wang XZ,et al. Layer-by-layer assembly of multilayer films composed of avidin and biotin-labeled antibody for immunosensing[J]. biosensors & bioelectronics,2003,18(1):59-67.
APA	Cui XQ.,Pei RJ.,Wang XZ.,Yang F.,Ma Y.,...&Yang XR.(2003).Layer-by-layer assembly of multilayer films composed of avidin and biotin-labeled antibody for immunosensing.biosensors & bioelectronics,18(1),59-67.
MLA	Cui XQ,et al."Layer-by-layer assembly of multilayer films composed of avidin and biotin-labeled antibody for immunosensing".biosensors & bioelectronics 18.1(2003):59-67.