Identification of Wheat-Barley 2H Alien Substitution Lines | |
Yuan Yaping1; Chen Xiao1; Xiao Shihe1; Islam A K M R2; Xin Zhiyong1 | |
刊名 | Acta Botanica Sinica |
2003 | |
卷号 | 45期号:9页码:1096-1102 |
关键词 | wheat barley substitution line genomic in situ hybridization (GISH) RFLP |
ISSN号 | 1672-6650 |
其他题名 | 小麦-大麦2H异代换系的鉴定 |
英文摘要 | The genetic constitution of fifteen materials derived from the cross wheat (Triticum aestivum L. cv. "Chinese Spring") * barley (Hordeum vulgare L. cv. "Betzes") was analyzed, and six disomic alien substitution lines were screened by GISH. The chromosome configurations in pollen mother cells at meiotic metaphaseⅠ(PMCs MⅠ) of F1 from each disomic substitution line respectively crossed with double ditelocentric lines 2A, 2B and 2D of "Chinese Spring" were observed, and a set of wheat-barley disomic alien substitution lines 2H(A), 2H(B) and 2H(D) were obtained. The RFLP analysis with the probe psr131 on the short arm of wheat homeologous group 2 combining with four restriction enzymes were carried out. The results indicated that the probe psr131 could be used as molecular marker to tag the barley chromosome 2H. The barley chromosome 2H had good genetic compensation ability for wheat chromosomes 2B and 2D in vitality and other agronomic characters. The result of testing seed was that the wheat appearance starch quality had been changed from the half-farinaceous of "Chinese Spring" to the half-cutin of substitution lines by transferring the barley chromosome 2H to wheat. |
学科主题 | 植物学 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.nais.net.cn/handle/2HMLN22E/131296] |
专题 | 作物科学研究所 |
作者单位 | 1.Institute of Crop Breeding and Cultivation, The Chinese Academy of Agricultural Sciences, Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, 北京, 100081; 2.Department of Plant Science, The University of Adelaide, Waite Campus, Glen Osmond, SA5064, 澳大利亚 |
推荐引用方式 GB/T 7714 | Yuan Yaping,Chen Xiao,Xiao Shihe,et al. Identification of Wheat-Barley 2H Alien Substitution Lines[J]. Acta Botanica Sinica,2003,45(9):1096-1102. |
APA | Yuan Yaping,Chen Xiao,Xiao Shihe,Islam A K M R,&Xin Zhiyong.(2003).Identification of Wheat-Barley 2H Alien Substitution Lines.Acta Botanica Sinica,45(9),1096-1102. |
MLA | Yuan Yaping,et al."Identification of Wheat-Barley 2H Alien Substitution Lines".Acta Botanica Sinica 45.9(2003):1096-1102. |
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