Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae

doi:10.1002/pmic.201100062

CORC > 中国农业科学院 > 植物保护研究所

	Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae
	Wang, Fu-Xin 1,2; Ma, Yin-Ping 1,2; Yang, Chun-Lin 1,2; Zhao, Pi-Ming 1,2; Yao, Yuan 1,2; Jian, Gui-Liang 3; Luo, Yuan-Ming 1,4; Xia, Gui-Xian 1,2
刊名	PROTEOMICS
	2011
卷号	11 期号:22 页码:4296-4309
关键词	Differential 2-D SDS-PAGE Gossypium barbadense Mass spectrometry Plant proteomics Verticillium dahliae
ISSN号	1615-9853
DOI	10.1002/pmic.201100062
通讯作者	Wang, Fu-Xin
英文摘要	Verticillium wilt of cotton is a vascular disease mainly caused by the soil-born filamentous fungus Verticillium dahliae. To study the mechanisms associated with defense responses in wilt-resistant sea-island cotton (Gossypium barbadense) upon V. dahliae infection, a comparative proteomic analysis between infected and mock-inoculated roots of G. barbadense var. Hai 7124 (a cultivar showing resistance against V. dahliae) was performed by 2-DE combined with local EST database-assisted PMF and MS/MS analysis. A total of 51 upregulated and 17 downregulated proteins were identified, and these proteins are mainly involved in defense and stress responses, primary and secondary metabolisms, lipid transport, and cytoskeleton organization. Three novel clues regarding wilt resistance of G. barbadense are gained from this study. First, ethylene signaling was significantly activated in the cotton roots attacked by V. dahliae as shown by the elevated expression of ethylene biosynthesis and signaling components. Second, the Bet v 1 family proteins may play an important role in the defense reaction against Verticillium wilt. Third, wilt resistance may implicate the redirection of carbohydrate flux from glycolysis to pentose phosphate pathway (PPP). To our knowledge, this study is the first root proteomic analysis on cotton wilt resistance and provides important insights for establishing strategies to control this disease.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; BIOCHEMICAL RESEARCH METHODS ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000297560300002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12324]
专题	植物保护研究所
作者单位	1.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China 2.State Key Lab Plant Genom, Beijing, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China 4.State Key Lab Microbial Resources, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fu-Xin,Ma, Yin-Ping,Yang, Chun-Lin,et al. Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae[J]. PROTEOMICS,2011,11(22):4296-4309.
APA	Wang, Fu-Xin.,Ma, Yin-Ping.,Yang, Chun-Lin.,Zhao, Pi-Ming.,Yao, Yuan.,...&Xia, Gui-Xian.(2011).Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae.PROTEOMICS,11(22),4296-4309.
MLA	Wang, Fu-Xin,et al."Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae".PROTEOMICS 11.22(2011):4296-4309.