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	Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1
	Ma, Gui-Zhen1; Gao, Hui-Lan2; Zhang, Yong-Hua2; Li, Shi-Dong2; Xie, Bing-Yan3; Wu, Sheng-Jun1
刊名	AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
	2012
卷号	6期号:20页码:4377-4383
关键词	Gliocladium catenulatum chitinases purification characteristics
ISSN号	1996-0808
DOI	10.5897/AJMR12.605
通讯作者	Ma, Gui-Zhen
英文摘要	Chitinase was isolated and purified from Gliocladium catenulatum through ammonium sulfate sedimentation, sephadex G-25 gel filtration, deionization, ultra filtration condensation, negative ion exchange separation, and non-denaturing gel electrophoresis. A 51 kDa chitinase was purified from the fungus through sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum temperature and pH for chitinase activity were 60 degrees C and pH 6.0, respectively. The enzyme solution was stable from 20 to 40 degrees C and pH 4 to 5. Co2+ and Ca2+ were conducive to the enzyme activity, whereas the Fe3+, Cu2+, and Ag+ evidently inhibited the enzyme activity. Fe2+, Na+, K+, Zn2+, and Mn2+ showed less inhibitory effects on the enzyme activity. The K-m of the chitinase was 2.832 mg ml(-1). The chitanase were found to inhibit the hyphal growth, conidial germination and sclerotial germination of various plant pathogenic fungi.
学科主题	Microbiology
语种	英语
出版者	ACADEMIC JOURNALS
WOS记录号	WOS:000307405500025
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12258]
专题	植物保护研究所
作者单位	1.Huaihai Inst Technol, Lianyungang 222005, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetable & Flower, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Ma, Gui-Zhen,Gao, Hui-Lan,Zhang, Yong-Hua,et al. Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2012,6(20):4377-4383.
APA	Ma, Gui-Zhen,Gao, Hui-Lan,Zhang, Yong-Hua,Li, Shi-Dong,Xie, Bing-Yan,&Wu, Sheng-Jun.(2012).Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1.AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,6(20),4377-4383.
MLA	Ma, Gui-Zhen,et al."Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 6.20(2012):4377-4383.

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