Diverse genetic basis of field-evolved resistance to Bt cotton in cotton bollworm from China

doi:10.1073/pnas.1200156109

CORC > 中国农业科学院 > 植物保护研究所

	Diverse genetic basis of field-evolved resistance to Bt cotton in cotton bollworm from China
	Zhang, Haonan 1; Tian, Wen 1; Zhao, Jing 1; Jin, Lin 1; Yang, Jun 1; Liu, Chunhui 1; Yang, Yihua 1; Wu, Shuwen 1; Wu, Kongming 2; Cui, Jinjie 3
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2012
卷号	109 期号:26 页码:10275-10280
关键词	F-1 screen F-2 screen dominant resistance
ISSN号	0027-8424
DOI	10.1073/pnas.1200156109
通讯作者	Zhang, Haonan
英文摘要	Evolution of pest resistance reduces the efficacy of insecticidal proteins from Bacillus thuringiensis (Bt) used in sprays or in transgenic crops. Although several pests have evolved resistance to Bt crops in the field, information about the genetic basis of field-evolved resistance to Bt crops has been limited. In particular, laboratory-selected resistance to Bt toxin Cry1Ac based on recessive mutations in a gene encoding a toxin-binding cadherin protein has been identified in three major cotton pests, but previous work has not determined if such mutations are associated with field-selected resistance to Bt cotton. Here we show that the most common resistance alleles in field populations of cotton bollworm, Helicoverpa armigera, selected with Bt cotton in northern China, had recessive cadherin mutations, including the deletion mutation identified via laboratory selection. However, unlike all previously studied cadherin resistance alleles, one field-selected cadherin resistance allele conferred nonrecessive resistance. We also detected nonrecessive resistance that was not genetically linked with the cadherin locus. In field-selected populations, recessive cadherin alleles accounted for 75-84% of resistance alleles detected. However, most resistance alleles occurred in heterozygotes and 59-94% of resistant individuals carried at least one nonrecessive resistance allele. The results suggest that resistance management strategies must account for diverse resistance alleles in field-selected populations, including nonrecessive alleles.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000306291400043
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12249]
专题	植物保护研究所
作者单位	1.Nanjing Agr Univ, Coll Plant Protect, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China 3.Chinese Acad Agr Sci, Inst Cotton Res, Anyang 455000, Peoples R China 4.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA
推荐引用方式 GB/T 7714	Zhang, Haonan,Tian, Wen,Zhao, Jing,et al. Diverse genetic basis of field-evolved resistance to Bt cotton in cotton bollworm from China[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(26):10275-10280.
APA	Zhang, Haonan.,Tian, Wen.,Zhao, Jing.,Jin, Lin.,Yang, Jun.,...&Wu, Yidong.(2012).Diverse genetic basis of field-evolved resistance to Bt cotton in cotton bollworm from China.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,109(26),10275-10280.
MLA	Zhang, Haonan,et al."Diverse genetic basis of field-evolved resistance to Bt cotton in cotton bollworm from China".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.26(2012):10275-10280.