Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor

doi:10.1007/s11033-012-2165-z

CORC > 中国农业科学院 > 植物保护研究所

	Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor
	Li, Yan 1; Sun, Yan 2; Yang, Qingchuan 1; Fang, Feng 3; Kang, Junmei 1; Zhang, Tiejun 1
刊名	MOLECULAR BIOLOGY REPORTS
	2013
卷号	40 期号:2 页码:1227-1239
关键词	Medicago Sativa L Gene cloning Subcellular localization Real time fluorescent quantitative PCR analysis Transgenic tobacco analysis
ISSN号	0301-4851
DOI	10.1007/s11033-012-2165-z
通讯作者	Li, Yan
英文摘要	A full-length cDNA of 1,537 nucleotides was cloned from Medicago sativa L. cv. "Zhongmu No. 1" by rapid amplification of cDNA ends. It was designated as MsZIP, encoding a protein of 340 amino acids. The protein molecular weight was 36.43 kDa, and the theoretical isoelectric point was 5.72. The MsZIP preferentially localized in nucleus and have signal peptide. Blast analysis revealed that MsZIP shared the highest homology with some bZIP proteins of M. truncatula. The transcript of MsZIP was strongly enriched in leaf compared with root and stem of mature alfalfa plants. MsZIP was strongly induced by 15 % PEG6000 (polyethylene glycol), 50 mu M abscisic acid, 200 mM NaCl, 70 mu M gibberellic acid, 5 mM salicylic acid and 200 mu M methyl jasmonate. Physiological resistance parameters were measured in the transgenic tobacco. Malondialdehyde content, relative water content, soluble sugar content, soluble protein content and proline content in transgenic tobacco increased compared with non-transgenic tobacco under salt stress or drought stress. The results showed that accumulation of the MsZIP protein in the vegetative tissues of transgenic plants enhanced their tolerance to osmotic pressure stress. These results demonstrate a role for the MsZIP protein in stress protection and suggest the potential of the MsZIP gene for genetic engineering of salt tolerance and drought tolerance.
学科主题	Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000313165500053
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12170]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 2.China Agr Univ, Inst Grassland Sci, Beijing 100193, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, Yan,Sun, Yan,Yang, Qingchuan,et al. Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor[J]. MOLECULAR BIOLOGY REPORTS,2013,40(2):1227-1239.
APA	Li, Yan,Sun, Yan,Yang, Qingchuan,Fang, Feng,Kang, Junmei,&Zhang, Tiejun.(2013).Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor.MOLECULAR BIOLOGY REPORTS,40(2),1227-1239.
MLA	Li, Yan,et al."Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor".MOLECULAR BIOLOGY REPORTS 40.2(2013):1227-1239.