Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China

doi:10.1007/s10658-013-0249-9

CORC > 中国农业科学院 > 植物保护研究所

	Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China
	Pan, Z. C.1,3; Xu, J.1; Prior, P.2; Xu, J. S.1; Zhang, H.1; Chen, K. Y.1; Tian, Q.1; Zhang, L. Q.1; Liu, L.1; He, L. Y.1
刊名	EUROPEAN JOURNAL OF PLANT PATHOLOGY
	2013
卷号	137 期号:2 页码:377-391
关键词	Mulberry Ralstonia solanacearum Detection Suppression subtractive hybridization Insertion sequences
ISSN号	0929-1873
DOI	10.1007/s10658-013-0249-9
通讯作者	Pan, Z. C.
英文摘要	Ralstonia solanacearum causes bacterial wilt disease in many plant species, including mulberry. Here, we used a suppression subtractive hybridization (SSH) approach to identify specific DNA fragments in R. solanacearum race 5-biovar 5. The genome of the R. solanacearum M7 strain was subtracted from that of the GMI1000 strain, resulting in the identification of 85 subtracted fragments. The primer set MG67-F/R for identification of Ralstonia solanacearum race 5-biovar 5 strains was designed on the basis of the clone MG67 sequence. Furthermore, a multiplex PCR was developed by using the primer set MG67-F/MG67R in combination with the species-specific primer pair 759/760. A 156 bp r5-bv5-specific fragment, together with a 282 bp species-specific fragment, was amplified from all tested R. solanacearum r5-bv5 strains. The sensitivity of the multiplex PCR made it possible to detect concentrations as low as 10(2) CFU ml(-1) of pure culture. Moreover, the r5-bv5-specific multiplex PCR was successfully applied to detect Ralstonia solanacearum race 5-biovar 5 strains in diseased mulberry samples. Therefore, the multiplex PCR assay can be used as a reliable diagnostic technique to enable researchers to rapidly identify isolates of R. solanacearum race 5-biovar 5.
学科主题	Agronomy ; Plant Sciences ; Horticulture ; AGRONOMY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000323747800017
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12088]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.CIRAD INRA, UMRC53, Plant Communities & Biol Invaders Trop Climates P, St Pierre 97410, Reunion 3.Yunnan Acad Agr Sci, Ind Crop Res Inst, Yunnan Potato Engn & Technol Res Ctr, Natl Potato Improvement Ctr,Yunnan Branch, Kunming 650200, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Pan, Z. C.,Xu, J.,Prior, P.,et al. Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China[J]. EUROPEAN JOURNAL OF PLANT PATHOLOGY,2013,137(2):377-391.
APA	Pan, Z. C..,Xu, J..,Prior, P..,Xu, J. S..,Zhang, H..,...&Feng, J..(2013).Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China.EUROPEAN JOURNAL OF PLANT PATHOLOGY,137(2),377-391.
MLA	Pan, Z. C.,et al."Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China".EUROPEAN JOURNAL OF PLANT PATHOLOGY 137.2(2013):377-391.