Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals

doi:10.1016/j.jinsphys.2013.10.006

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	Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals
	Zhou, Yan-Le 1; Zhu, Xiao-Qiang 1; Gu, Shao-Hua 1; Cui, Huan-huan 1; Guo, Yu-Yuan 1; Zhou, Jing-Jiang 2; Zhang, Yong-Jun 1
刊名	JOURNAL OF INSECT PHYSIOLOGY
	2014
卷号	60 页码:31-39
关键词	Apolygus lucorum Olfactory coreceptor Expression profile Electroantennogram RNA interference
ISSN号	0022-1910
DOI	10.1016/j.jinsphys.2013.10.006
通讯作者	Zhou, Yan-Le
英文摘要	Apolygus lucorum (Meyer-Dur) (Hemiptera: Miridae) is an agronomically important pest that causes severe economic damage to the cotton, fruit, and vegetable industries. Similar to other insects, A. lucorum can perceive and discriminate olfactory cues. A highly conserved and broadly expressed olfactory coreceptor (Orco) is crucial for insect olfaction, and Orco orthologs have been identified in several insect species. In this study, a homology-based polymerase chain reaction (PCR) method was utilized to identify AlucOrco, an Orco ortholog essential for olfaction in A. lucorum. AlucOrco shares significant sequence homology with known Orco proteins in other insects. Quantitative real-time PCR (qRT-PCR) analysis revealed that AlucOrco was abundantly expressed in adult A. lucorum. AlucOrco expression level was the highest in the antennae; by contrast, AlucOrco showed negligible expression level in other tissues. We injected AlucOrco siRNA into the conjunctivum between the prothorax and mesothorax of A. lucorum and evaluated its expression 36 h after RNA interference. The results of qRT-PCR demonstrated that the level of mRNA expression was significantly reduced (>90%) in AlucOrco siRNA-treated A. lucorum than in water-injected and non-injected controls. The electroantennogram responses of A. lucorum to two putative semiochemicals, trans-2-hexenal and trans-2-hexenyl butyrate, were also reduced significantly (similar to 80%) in RNAi-treated A. lucorum than in the controls. These results suggest that AlucOrco is crucial in mediating odorant perception of A. lucorum, especially in perceiving trans-2-hexenal and trans-2-hexenyl butyrate. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Entomology ; Physiology ; Zoology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000330747000005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/12061]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Rothamsted Res, Dept Biol Chem, Harpenden AL5 2JQ, Herts, England
推荐引用方式 GB/T 7714	Zhou, Yan-Le,Zhu, Xiao-Qiang,Gu, Shao-Hua,et al. Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals[J]. JOURNAL OF INSECT PHYSIOLOGY,2014,60:31-39.
APA	Zhou, Yan-Le.,Zhu, Xiao-Qiang.,Gu, Shao-Hua.,Cui, Huan-huan.,Guo, Yu-Yuan.,...&Zhang, Yong-Jun.(2014).Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals.JOURNAL OF INSECT PHYSIOLOGY,60,31-39.
MLA	Zhou, Yan-Le,et al."Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals".JOURNAL OF INSECT PHYSIOLOGY 60(2014):31-39.