Large-scale identification of microsatellites for a major invasive weed, Ageratina adenophora, using the Illumina sequencing technology

doi:10.1111/wre.12064

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	Large-scale identification of microsatellites for a major invasive weed, Ageratina adenophora, using the Illumina sequencing technology
	Nie, X. J.1,2; Liu, P. X.1,2; Deng, P. C.1,2; Lu, Y. Z.1,2; Biradar, S. S.1,2; Du, X. H.1,2; Wu, A. P.3; Wan, F. H.3; Weining, S.1,2
刊名	WEED RESEARCH
	2014
卷号	54 期号:2 页码:133-139
关键词	invasive weed next-generation sequencing Illumina sequencing crofton weed molecular marker microsatellites
ISSN号	0043-1737
DOI	10.1111/wre.12064
通讯作者	Nie, X. J.
英文摘要	Microsatellites were identified and characterised for a worldwide invasive weed, Ageratina adenophora, based on the Illumina sequencing technology. Using the assembled genomic sequences obtained from the Illumina GAII sequencing platform, a total of 3012 microsatellites were identified, of which mono-, di-, tri-, tetra-, penta-, and hexa-nucleotide repeats were 181 (6.0%), 1379 (45.8%), 1064 (35.3%), 220 (7.3%), 73 (2.4%) and 95 (3.2%) respectively. The AC/GT and GAT/ATC motifs were the most frequent motif types of di-nucleotide and tri-nucleotide repeats respectively. To validate the newly identified simple sequence repeat markers, 15 primer pairs were randomly selected to test in 24 individuals of A.adenophora, and all of these primer sets could amplify PCR products of expected size. Of them, eight loci displayed polymorphism with the number of alleles per locus ranging from two to four and values for observed and expected heterozygosities ranging from 0.042 to 1 and from 0.082 to 0.551, with the averages of 0.578 and 0.371 respectively. These novel microsatellites should be useful for investigation of the genetic structure and genetic diversity between the invasive and native populations of this species.
学科主题	Agronomy ; Plant Sciences ; AGRONOMY ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000332071500003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11979]
专题	植物保护研究所
作者单位	1.Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China 2.Northwest A&F Univ, China Wheat Improvement Ctr, Yangling Branch, Yangling 712100, Shaanxi, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Nie, X. J.,Liu, P. X.,Deng, P. C.,et al. Large-scale identification of microsatellites for a major invasive weed, Ageratina adenophora, using the Illumina sequencing technology[J]. WEED RESEARCH,2014,54(2):133-139.
APA	Nie, X. J..,Liu, P. X..,Deng, P. C..,Lu, Y. Z..,Biradar, S. S..,...&Weining, S..(2014).Large-scale identification of microsatellites for a major invasive weed, Ageratina adenophora, using the Illumina sequencing technology.WEED RESEARCH,54(2),133-139.
MLA	Nie, X. J.,et al."Large-scale identification of microsatellites for a major invasive weed, Ageratina adenophora, using the Illumina sequencing technology".WEED RESEARCH 54.2(2014):133-139.