Sphaerisporangium dianthi sp nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L

doi:10.1007/s10482-014-0298-x

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	Sphaerisporangium dianthi sp nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L
	Xing, Jia 1; Liu, Chongxi 1; Zhang, Yuejing 1; He, Hairong 2; Zhou, Ying 1; Li, Lianjie 1; Zhao, Junwei 1; Liu, Shuanghe 1; Wang, Xiangjing 1; Xiang, Wensheng 1,2
刊名	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
	2015
卷号	107 期号:1 页码:9-14
关键词	Sphaerisporangium dianthi sp nov. Polyphasic taxonomy 16S rRNA gene
ISSN号	0003-6072
DOI	10.1007/s10482-014-0298-x
通讯作者	Xing, Jia
英文摘要	A novel actinomycete, designated strain NEAU-CY18(T), was isolated from the root of a Chinese medicinal plant Dianthus chinensis L and subjected to a polyphasic taxonomic study. The novel strain was found to develop spherical sporangia with non-motile spores on aerial mycelium. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The whole-cell sugars were identified as madurose, mannose, ribose, galactose and glucose. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were identified as MK-9(H-4), MK-9(H-2) and MK-9(H-6). The major fatty acids were identified as C-17:0 10-methyl, iso-C-16:0 and C-16:0. EzTaxon-e analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Sphaerisporangium and was most closely related to Sphaerisporangium cinnabarinum JCM 3291(T) (98.9 %) and Sphaerisporangium melleum JCM 13064(T) (98.3 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-CY18(T) forms a monophyletic clade with S. cinnabarinum JCM 3291(T), an association that was supported by a bootstrap value of 97 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. Comparisons of some phenotypic properties and low DNA-DNA relatedness values enabled the strain to be differentiated from S. cinnabarinum JCM 3291(T) and S. melleum JCM 13064(T). Therefore, it is concluded that strain NEAU-CY18(T) represents a novel Sphaerisporangium species, for which the name Sphaerisporangium dianthi sp. nov. is proposed. The type strain is NEAU-CY18(T) ( = CGMCC 4.7132(T) = DSM 46736(T)).
学科主题	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000347249500002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11814]
专题	植物保护研究所
作者单位	1.Northeast Agr Univ, Heilongjiang Prov Educ Comm, Key Lab Agr Biol Funct Gene, Harbin 150030, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Xing, Jia,Liu, Chongxi,Zhang, Yuejing,et al. Sphaerisporangium dianthi sp nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2015,107(1):9-14.
APA	Xing, Jia.,Liu, Chongxi.,Zhang, Yuejing.,He, Hairong.,Zhou, Ying.,...&Xiang, Wensheng.(2015).Sphaerisporangium dianthi sp nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,107(1),9-14.
MLA	Xing, Jia,et al."Sphaerisporangium dianthi sp nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 107.1(2015):9-14.