Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)

doi:10.1038/srep08073

CORC > 中国农业科学院 > 植物保护研究所

	Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)
	Sun, Liang 1,2,3; Zhou, Jing-Jiang 4; Gu, Shao-Hua 1; Xiao, Hai-Jun 1,5; Guo, Yu-Yuan 1; Liu, Ze-Wen 3; Zhang, Yong-Jun 1
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:-
ISSN号	2045-2322
DOI	10.1038/srep08073
通讯作者	Sun, Liang
英文摘要	Insect chemosensory proteins (CSPs) are a family of small soluble proteins. To date, their physiological functions in insect olfaction remain largely controversial in comparison to odorant binding proteins (OBPs). In present study, we reported the antenna specific expression of three CSPs (AlinCSP4-6) from Adelphocoris lineolatus, their distinct chemosensillum distribution as well as ligand binding capability thus providing the evidence for the possible roles that they could play in semiochemical detection of the plant bug A. lineolatus. The results of qRT-PCR and western blot assay clearly showed that all of these three CSPs are highly expressed in the adult antennae, the olfactory organ of insects. Further cellular investigation of their immunolocalization revealed their dynamic protein expression profiles among different types of antennal sensilla. In a fluorescence competitive binding assay, the selective ligand binding was observed for AlinCSP4-6. In addition, a cooperative interaction was observed between two co-expressed CSPs resulting in an increase of the binding affinities by a mixture of AlinCSP5 and AlinCSP6 to terpenoids which do not bind to individual CSPs. These findings in combination with our previous data for AlinCSP1-3 indicate a possible functional differentiation of CSPs in the A. lineolatus olfactory system.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000348435800008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11791]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Tea Res Inst, Key Lab Tea Plants Biol & Resources Utilizat, Minist Agr, Hangzhou 310008, Zhejiang, Peoples R China 3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing 210095, Jiangsu, Peoples R China 4.Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England 5.Jiangxi Agr Univ, Inst Entomol, Nanchang 330045, Peoples R China
推荐引用方式 GB/T 7714	Sun, Liang,Zhou, Jing-Jiang,Gu, Shao-Hua,et al. Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)[J]. SCIENTIFIC REPORTS,2015,5:-.
APA	Sun, Liang.,Zhou, Jing-Jiang.,Gu, Shao-Hua.,Xiao, Hai-Jun.,Guo, Yu-Yuan.,...&Zhang, Yong-Jun.(2015).Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze).SCIENTIFIC REPORTS,5,-.
MLA	Sun, Liang,et al."Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)".SCIENTIFIC REPORTS 5(2015):-.