Detection of Tilletia controversa using immunofluorescent monoclonal antibodies

doi:10.1111/jam.12703

CORC > 中国农业科学院 > 植物保护研究所

	Detection of Tilletia controversa using immunofluorescent monoclonal antibodies
	Gao, L.1; Feng, C.2; Li, B.3; Liu, T.1; Liu, B.1; Chen, W.1
刊名	JOURNAL OF APPLIED MICROBIOLOGY
	2015
卷号	118 期号:2 页码:497-505
关键词	detection immunofluorescence method monoclonal antibody plant pathology Tilletia controversa wheat smut
ISSN号	1364-5072
DOI	10.1111/jam.12703
通讯作者	Gao, L.
英文摘要	AimsTilletia controversa is an internationally quarantined pathogenic fungus that causes dwarf bunt of wheat and is similar to Tilletia caries in both teliospore morphology and genetic structure. This study developed a rapid and sensitive immunofluorescence method for differentiating the teliospores of T.controversa from T.caries.Methods and ResultsThe method utilizes monoclonal antibody D-1 against teliospores of T.controversa as well as a PE-Cy3-conjugated goat anti-mouse antibody (overlapping light excitation of 495 and 555nm). The orange cycle fluorescent signal was stronger against T.controversa teliospores in the outer spore wall and net ridge, whereas only the green signal was observed for the protoplasm of T.caries teliospores. The detection limit of this method was 20gml(-1) of the D-1 monoclonal antibody.ConclusionThis study describes the production and diagnostic application of a novel mouse monoclonal antibody specific to T.controversa teliospores.Significance and Impact of the StudyThis method could be used for the on-site identification of T.controversa teliospores in the near future and will help in selecting fungicides to control dwarf bunt of wheat as further technical developments are achieved.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000348854500021
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11786]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Beijing Kwinbon Biotechnol, Int Informat Ind Base, Beijing, Peoples R China 3.Beijing Ctr Phys & Chem Anal, Yongfeng High & New Technol Ind Base, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gao, L.,Feng, C.,Li, B.,et al. Detection of Tilletia controversa using immunofluorescent monoclonal antibodies[J]. JOURNAL OF APPLIED MICROBIOLOGY,2015,118(2):497-505.
APA	Gao, L.,Feng, C.,Li, B.,Liu, T.,Liu, B.,&Chen, W..(2015).Detection of Tilletia controversa using immunofluorescent monoclonal antibodies.JOURNAL OF APPLIED MICROBIOLOGY,118(2),497-505.
MLA	Gao, L.,et al."Detection of Tilletia controversa using immunofluorescent monoclonal antibodies".JOURNAL OF APPLIED MICROBIOLOGY 118.2(2015):497-505.