Rhizosphere Inhibition of Cucumber Fusarium Wilt by Different Surfactin-excreting Strains of Bacillus subtilis

doi:10.5423/PPJ.OA.10.2014.0113

CORC > 中国农业科学院 > 植物保护研究所

	Rhizosphere Inhibition of Cucumber Fusarium Wilt by Different Surfactin-excreting Strains of Bacillus subtilis
	Jia, Ke 1; Gao, Yu-Han 1; Huang, Xiao-Qin 2; Guo, Rong-Jun 1; Li, Shi-Dong 1
刊名	PLANT PATHOLOGY JOURNAL
	2015
卷号	31 期号:2 页码:140-151
关键词	colonization Fusarium oxysporum f. sp cucumerinum real-time PCR surfactin
ISSN号	1598-2254
DOI	10.5423/PPJ.OA.10.2014.0113
通讯作者	Jia, Ke
英文摘要	Bacillus subtilis B006 strain effectively suppresses the cucumber fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (Foc). The population dynamics of Foc, strain B006 and its surfactin over-producing mutant B841 and surfactin-deficient mutant B1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopeptides excreted by these strains in suppressing Foe. Results showed that B. subtilis strain B006 effectively suppressed the disease in natural soil by 42.9%, five weeks after transplanting, whereas B841 and B1020 suppressed the disease by only 22.6% and 7.1%, respectively. Quantitative PCR assays showed that effective colonization of strain B006 in the rhizosphere suppressed Foe propagation by more than 10 times both in nursery substrate and in field-infected soil. Reduction of Foe population at the cucumber stems in a range of 0.96 log(10) ng/g to 2.39 log(10) ng/gwas attained at the third and the fifth weeks of B006 treatment in nursery substrate. In field-infected soil, all three treatments with B. subtilis suppressed Foe infection, indicated by the reduction of Foe population at a range of 2.91 log(10) ng/g to 3.36 log(10) ng/g at the stem base, one week after transplanting. This study reveals that the suppression of fusarium wilt disease is affected by the effective colonization of the surfactin-producing B. subtilis strain in the rhizosphere. These results improved our understanding of the biocontrol mechanism of the B. subtilis strain B006 in the natural soil and facilitate its application as biocontrol agent in the field.
学科主题	Agriculture, Multidisciplinary ; Plant Sciences
语种	英语
出版者	KOREAN SOC PLANT PATHOLOGY
WOS记录号	WOS:000355499100006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11712]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, Minist Agr, Key Lab Pest Management Crops, Beijing 100081, Peoples R China 2.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China
推荐引用方式 GB/T 7714	Jia, Ke,Gao, Yu-Han,Huang, Xiao-Qin,et al. Rhizosphere Inhibition of Cucumber Fusarium Wilt by Different Surfactin-excreting Strains of Bacillus subtilis[J]. PLANT PATHOLOGY JOURNAL,2015,31(2):140-151.
APA	Jia, Ke,Gao, Yu-Han,Huang, Xiao-Qin,Guo, Rong-Jun,&Li, Shi-Dong.(2015).Rhizosphere Inhibition of Cucumber Fusarium Wilt by Different Surfactin-excreting Strains of Bacillus subtilis.PLANT PATHOLOGY JOURNAL,31(2),140-151.
MLA	Jia, Ke,et al."Rhizosphere Inhibition of Cucumber Fusarium Wilt by Different Surfactin-excreting Strains of Bacillus subtilis".PLANT PATHOLOGY JOURNAL 31.2(2015):140-151.