Molecular Characterization and Expression Profiling of Odorant-Binding Proteins in Apolygus lucorum

doi:10.1371/journal.pone.0140562

CORC > 中国农业科学院 > 植物保护研究所

	Molecular Characterization and Expression Profiling of Odorant-Binding Proteins in Apolygus lucorum
	Yuan, Hai-Bin 1,2; Ding, Yu-Xiao 1,2; Gu, Shao-Hua 2; Sun, Liang 3; Zhu, Xiao-Qiang 2; Liu, Hang-Wei 2; Dhiloo, Khalid Hussain 2,4; Zhang, Yong-Jun 2; Guo, Yu-Yuan 2
刊名	PLOS ONE
	2015
卷号	10 期号:10 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0140562
通讯作者	Yuan, Hai-Bin
英文摘要	Apolygus lucorum (Meyer-Dur) (Hemiptera: Miridae) is one of the most important agricultural pests, with broad host range and cryptic feeding habits in China. Chemosensory behavior plays an important role in many crucial stages in the life of A. lucorum, such as the detection of sex pheromone cues during mate pursuit and fragrant odorants during flowering host plant localization. Odorant-binding proteins (OBPs) are involved in the initial biochemical recognition steps in semiochemical perception. In the present study, a transcriptomics-based approach was used to identify potential OBPs in A. lucorum. In total, 38 putative OBP genes were identified, corresponding to 26 'classic' OBPs and 12 'Plus-C' OBPs. Phylogenetic analysis revealed that A. lucorum OBP proteins are more closely related to the OBP proteins of other mirid bugs as the same family OBP clustering together. Quantitative real-time PCR analysis for the first reported 23 AlucOBPs revealed that the expression level of 11 AlucOBP genes were significantly higher in antennae of both sexes than in other tissues. Three of them were male antennae-biased and six were female antennae-biased, suggesting their putative roles in the detection of female sex pheromones and host plant volatiles. In addition, three, four, two and one AlucOBPs had the highest degree of enrichment in the stylet, head, leg, and in abdomen tissues, respectively. Two other OBPs were ubiquitously expressed in the main tissues, including antennae, stylets, heads, legs and wings. Most orthologs had similar expression patterns, strongly indicating that these genes have the same function in olfaction and gustation.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000363183100146
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11623]
专题	植物保护研究所
作者单位	1.Jilin Agr Univ, Coll Agron, Changchun 130118, Jilin, Peoples R China 2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 3.Chinese Acad Agr Sci, Tea Res Inst, Key Lab Tea Plants Biol & Resources Utilizat, Minist Agr, Hangzhou 310008, Zhejiang, Peoples R China 4.Sindh Agr Univ Tandojam, Fac Crop Protect, Dept Entomol, Tandojam, Pakistan
推荐引用方式 GB/T 7714	Yuan, Hai-Bin,Ding, Yu-Xiao,Gu, Shao-Hua,et al. Molecular Characterization and Expression Profiling of Odorant-Binding Proteins in Apolygus lucorum[J]. PLOS ONE,2015,10(10):-.
APA	Yuan, Hai-Bin.,Ding, Yu-Xiao.,Gu, Shao-Hua.,Sun, Liang.,Zhu, Xiao-Qiang.,...&Guo, Yu-Yuan.(2015).Molecular Characterization and Expression Profiling of Odorant-Binding Proteins in Apolygus lucorum.PLOS ONE,10(10),-.
MLA	Yuan, Hai-Bin,et al."Molecular Characterization and Expression Profiling of Odorant-Binding Proteins in Apolygus lucorum".PLOS ONE 10.10(2015):-.