Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China

doi:10.1016/S2095-3119(15)61175-3

CORC > 中国农业科学院 > 植物保护研究所

	Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China
	Qiu Cai-ling 1,2; Zhang Zhi-xiang 3; Li Shi-fang 3; Bai Yan-ju 1,2; Liu Shang-wu 2; Fan Guo-quan 2; Gao Yan-ling 1,2; Zhang Wei 1,2; Zhang Shu 2; Lu Wen-he 1
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2016
卷号	15 期号:2 页码:349-363
关键词	certification germplasm phylogenetic analysis quarantine seed potato viroid
ISSN号	2095-3119
DOI	10.1016/S2095-3119(15)61175-3
通讯作者	Qiu Cai-ling
英文摘要	China is the largest potato producing country worldwide, with this crop representing the fourth largest staple food crop in China. However, the steady presence of Potato spindle tuber viroid (PSTVd) over the past five decades has a significant economic impact on potato production. To determine why PSTVd control measures have been ineffective in China, more than 1 000 seed potatoes collected between 2009 and 2014 were subjected to PSTVd detection at the Supervision and Testing Center for Virus-free Seed Potatoes Quality, Ministry of Agriculture, China. A high PSTVd infection rate (6.5%) was detected among these commercial seed potatoes. Some breeding lines of potato collected from 2012 to 2015 were also tested for PSTVd infection, revealing a high rate of PSTVd contamination in these potato propagation materials. Furthermore, comparison of the full-length sequences of 71 different Chinese PSTVd isolates revealed a total of 74 predominant PSTVd variants, which represented 42 different sequence variants of PSTVd. Comparative sequence analysis revealed 30 novel PSTVd sequence variants specific to China. Comprehensive phylogenetic analysis uncovered a close relationship between the Chinese PSTVd sequence variants and those isolated from Russia. It is worth noting that three intermediate strains and six mild strains were identified among these variants. These results have important implications for explaining the ineffective control of PSTVd in China and thus could serve as a basic reference for designing more effective measures to eliminate PSTVd from China in the future.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000369551400011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11569]
专题	植物保护研究所
作者单位	1.Northeast Agr Univ, Harbin 150030, Peoples R China 2.Heilongjiang Acad Agr Sci, Virus Free Seedling Res Inst, Harbin 150086, Peoples R China 3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Qiu Cai-ling,Zhang Zhi-xiang,Li Shi-fang,et al. Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(2):349-363.
APA	Qiu Cai-ling.,Zhang Zhi-xiang.,Li Shi-fang.,Bai Yan-ju.,Liu Shang-wu.,...&Lu Dian-qiu.(2016).Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China.JOURNAL OF INTEGRATIVE AGRICULTURE,15(2),349-363.
MLA	Qiu Cai-ling,et al."Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China".JOURNAL OF INTEGRATIVE AGRICULTURE 15.2(2016):349-363.