Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae)

doi:10.1016/j.jinsphys.2016.05.002

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	Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae)
	Wang, Shan-Ning 1; Peng, Yong 2; Lu, Zi-Yun 3; Dhiloo, Khalid Hussain 1,4; Zheng, Yao 1; Shan, Shuang 1,5; Li, Rui-Jun 2; Zhang, Yong-Jun 1; Guo, Yu-Yuan 1
刊名	JOURNAL OF INSECT PHYSIOLOGY
	2016
卷号	90 页码:27-35
关键词	Microplitis mediator Antennae Ionotropic receptor Expression profile Olfactory sensory neuron
ISSN号	0022-1910
DOI	10.1016/j.jinsphys.2016.05.002
通讯作者	Wang, Shan-Ning
英文摘要	Ionotropic receptors (IRs) mainly detect the acids and amines having great importance in many insect species, representing an ancient olfactory receptor family in insects. In the present work, we performed RNAseq of Microplitis mediator antennae and identified seventeen IRs. Full-length MmedIRs were cloned and sequenced. Phylogenetic analysis of the Hymenoptera IRs revealed that ten MmedIR genes encoded "antennal IRs" and seven encoded "divergent IRs". Among the IR25a orthologous groups, two genes, MmedIR25a.1 and MmedIR25a.2, were found in M. mediator. Gene structure analysis of MmedIR25a revealed a tandem duplication of IR25a in M. mediator. The tissue distribution and development specific expression of the MmedIR genes suggested that these genes showed a broad expression profile. Quantitative gene expression analysis showed that most of the genes are highly enriched in adult antennae, indicating the candidate chemosensory function of this family in parasitic wasps. Using immunocytochemistry, we confirmed that one co-receptor, MmedlR8a, was expressed in the olfactory sensory neurons. Our data will supply fundamental information for functional analysis of the IRs in parasitoid wasp chemoreception. (C) 2016 Elsevier Ltd. All rights reserved.
学科主题	Entomology ; Physiology ; Zoology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000379095500004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11455]
专题	植物保护研究所
作者单位	1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China 2.Agr Univ Hebei, Coll Plant Protect, Baoding 071000, Peoples R China 3.Hebei Acad Agr & Forestry Sci, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov, Minist Agr,Plant Protect Inst, Baoding 071000, Hebei, Peoples R China 4.Sindh Agr Univ Tandojam, Fac Crop Protect, Dept Entomol, Tandojam, Pakistan 5.China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shan-Ning,Peng, Yong,Lu, Zi-Yun,et al. Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae)[J]. JOURNAL OF INSECT PHYSIOLOGY,2016,90:27-35.
APA	Wang, Shan-Ning.,Peng, Yong.,Lu, Zi-Yun.,Dhiloo, Khalid Hussain.,Zheng, Yao.,...&Guo, Yu-Yuan.(2016).Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae).JOURNAL OF INSECT PHYSIOLOGY,90,27-35.
MLA	Wang, Shan-Ning,et al."Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae)".JOURNAL OF INSECT PHYSIOLOGY 90(2016):27-35.