Lethal and Sub-Lethal Effects of Transgenic Rice Containing cry1Ac and CpTI Genes on the Pink Stem Borer,Sesamia inferens(Walker)

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	Lethal and Sub-Lethal Effects of Transgenic Rice Containing cry1Ac and CpTI Genes on the Pink Stem Borer,Sesamia inferens(Walker)
	HAN Lan-zhi 1; HOU Mao-lin 1; WU Kong-ming 1; PENG Yu-fa 1; WANG Feng 2
刊名	Agricultural Sciences in China
	2011
卷号	10 期号:3 页码:384-393
关键词	transgenic rice Sesamia inferens lethal and sub-lethal effect
ISSN号	1671-2927
英文摘要	Lethal and sub-lethal effects of transgenic rice containing cry1Ac and CpTI genes on the pink stem borer,Sesamia inferens,were studied to collect information for ecological risk assessment on insect-resistance of transgenic rice.In vitro insectfeeding bioassays were conducted to evaluate the lethal and sub-lethal effects of transgenic rice lines(II YouKF6 and KF6)containing cry1Ac+CpTI genes on S.inferens at four different growth stages,viz.,seedling,tillering and elongation,booting,and milk and maturing.Transgenic rice at seedling stage showed significantly high lethal effect on S.inferens with the shortest lethal duration for 50 and 100%individuals and the highest corrected mortalities after feeding on transgenic lines at this stage for 3 and 6 d.Followed by tillering and elongation stage,50 and 100%S.inferens were dead after feeding on transgenic lines at this stage for 4 and 10 d,respectively.Moreover,corrected mortalities for 6 d feeding on transgenic lines at this stage were significantly higher than that at booting,and milk and maturing stages.Lethal effect of KF6 on S.inferens decreased significantly at booting stage.Lethal duration for 50%S.inferens significantly extended and its corrected mortalities for 6 d feeding also declined remarkably.However,lethal effect of II YouKF6 on S.inferens did not decrease significantly at this stage.Transgenic rice at booting,and milk and maturing stages did not show significant lethal effect to S.inferens and it showed the longest lethal duration for 50%individuals and the lowest corrected mortalities for 3 and 6 d feeding.A few larvae of S.inferens could survive,pupate and emerge on these two transgenic lines at booting,and milk and maturing stages.Sub-lethal effect of two transgenic lines on S.inferens also differed significantly between different developmental stages.Continuously feeding on transgenic rice lines at seedling,and tillering and elongation stages delayed the development of larvae and pupae and decreased pupation rate,but no effect was observed on eclosion rate.Larval development was significantly inhibited after feeding on transgenic rice at booting stage,but no obvious effect was observed in pupal stage,pupation and eclosion rate.There were no significant differences for larval and pupal development,pupation,and eclosion rates between feeding on transgenic and control rice lines at milk and maturing stage.Larval and pupal weights significantly declined,but no influence was observed on fecundity when S.inferens infested on transgenic rice at any stage.These showed that there were significant differences in lethal and sub-lethal effects of transgenic rice on S.inferens among developmental stages,and the effects gradually decreased with the increase of growth stages of rice plant.
学科主题	植物保护
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11158]
专题	植物保护研究所
作者单位	1.Institute of Plant Protection,Chinese Academy of Agricultural Sciences, State Key Laboratory of Plant Disease and Insect Pests, Beijing, 100193 2.Fujian Academy of Agricultural Sciences, Key Laboratory of Agricultural Genetics Engineering,Fujian Academy of Agricultural Sciences, Fuzhou, 350003
推荐引用方式 GB/T 7714	HAN Lan-zhi,HOU Mao-lin,WU Kong-ming,et al. Lethal and Sub-Lethal Effects of Transgenic Rice Containing cry1Ac and CpTI Genes on the Pink Stem Borer,Sesamia inferens(Walker)[J]. Agricultural Sciences in China,2011,10(3):384-393.
APA	HAN Lan-zhi,HOU Mao-lin,WU Kong-ming,PENG Yu-fa,&WANG Feng.(2011).Lethal and Sub-Lethal Effects of Transgenic Rice Containing cry1Ac and CpTI Genes on the Pink Stem Borer,Sesamia inferens(Walker).Agricultural Sciences in China,10(3),384-393.
MLA	HAN Lan-zhi,et al."Lethal and Sub-Lethal Effects of Transgenic Rice Containing cry1Ac and CpTI Genes on the Pink Stem Borer,Sesamia inferens(Walker)".Agricultural Sciences in China 10.3(2011):384-393.