Transcriptome Analysis Revealing the Absence of Unintended Effects Both in Aerial and Underground Portions of Transgenic Rice (Oryza sativa L.) Huahui 1

doi:10.3969/j.issn.1674-7968.2015.07.004

CORC > 中国农业科学院 > 植物保护研究所

	Transcriptome Analysis Revealing the Absence of Unintended Effects Both in Aerial and Underground Portions of Transgenic Rice (Oryza sativa L.) Huahui 1
	Zhang Wenwei; Zhao Jie; Li Yunhe; Chen Xiuping; Jian Guiliang; Peng Yufa; Qi Fangjun
刊名	农业生物技术学报
	2015
卷号	23 期号:7 页码:876-887
关键词	Genetically modified rice Root Microarray analysis Differentially expressed genes Unintended effects
ISSN号	1674-7968
DOI	10.3969/j.issn.1674-7968.2015.07.004
其他题名	转录组分析转基因水稻华恢1号地上及地下部分不存在非预期效应
英文摘要	Unintended effects of genetically modified(GM) plants should be paid particular attention in the process of safety assessment of GM crops and their products, especially in regard to some long- term and potential food safety issues. To our knowledge, few research concerning on revealing possible unintended effects in underground portions of GM plants. In this study, with the aim to comprehensively investigate unintended effects of transgenic rice(Oryza sativa cv.) Huahui 1(HH1), we performed DNA microarray analysis both in aerial and underground portions of HH1 and compared to its non-transgenic parent(Oryza sativa) Minghui 63(MH63). The expression level of 8 genes located within 100 kb up and down-stream of insertion site of HH1 were not changed significantly. Considerable differentially expressed genes(DEGs) and significantly changed pathways were identified. The results showed that all these changes were not enough to cause obviously unintended effects both in aerial and underground portions of transgenic rice HH1. In addition, our study provides a clue to investigate unintended effects of GM plants in further safety assessment.; 从食品安全性考虑,必须关注转基因作物及其产品的非预期效应。迄今,很少有研究涉及转基因作物地下部分的非预期效应。本研究用转录组学方法比较分析了转基因水稻(Oryza sativa cv.)华恢1号及其对应亲本材料明恢63地上及地下部分可能存在的非预期效应。结果显示,华恢1号中外源基因插入位点上、下游100 kb内8个基因的表达水平没有显著性差异。虽可区分出转基因水稻与其亲本之间存在差异性的转录本和差异性表达的代谢途径,但这些差异均不足以引起转基因水稻地上和地下部分的非预期效应。本研究对进一步探索和发现转基因水稻的非预期效应并进行安全性评价提供了线索。
学科主题	农作物
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/11077]
专题	植物保护研究所
作者单位	Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing, 100193
推荐引用方式 GB/T 7714	Zhang Wenwei,Zhao Jie,Li Yunhe,et al. Transcriptome Analysis Revealing the Absence of Unintended Effects Both in Aerial and Underground Portions of Transgenic Rice (Oryza sativa L.) Huahui 1[J]. 农业生物技术学报,2015,23(7):876-887.
APA	Zhang Wenwei.,Zhao Jie.,Li Yunhe.,Chen Xiuping.,Jian Guiliang.,...&Qi Fangjun.(2015).Transcriptome Analysis Revealing the Absence of Unintended Effects Both in Aerial and Underground Portions of Transgenic Rice (Oryza sativa L.) Huahui 1.农业生物技术学报,23(7),876-887.
MLA	Zhang Wenwei,et al."Transcriptome Analysis Revealing the Absence of Unintended Effects Both in Aerial and Underground Portions of Transgenic Rice (Oryza sativa L.) Huahui 1".农业生物技术学报 23.7(2015):876-887.