Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees (Apis mellifera)

doi:10.1051/apido:2007059

	Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees (Apis mellifera)
	Li, Jianke 2; Feng, Mao 2; Zhang, Zhaohui 1; Pan, Yinghong 1
刊名	APIDOLOGIE
	2008
卷号	39 期号:2 页码:199-214
关键词	Apis mellifera winter bees hypopharyngeal gland proteome
ISSN号	0044-8435
DOI	10.1051/apido:2007059
通讯作者	Li, Jianke
英文摘要	We investigated the protein complement of the hypopharyngeal gland (HG) of winter worker bees from a strain of Apis mellifera artificially selected for increased royal jelly yield and A. m. carnica winter worker bees. Proteins were partially identified using two-dimensional gel electrophoresis (2-DE). MALDI-TOF MS and protein engine identification tools that were utilized for the honeybee genome. Most identified proteins in the two bees strains were assigned to major royal jelly (RJ) proteins (MRJPs). Marked differences were found in the heterogeneity of the MRJPs, in particular MRJP3. Two of the proteins, alpha-glucosidase and glucose oxidase, were related to carbohydrate metabolism and energy. For the first time in the HG of honeybees, two proteins, peroxiredoxin and thioredoxin peroxidase, which are related to antioxidation functions, and actin 5C, a major cytoskeletal actin protein which may supply enough actin for normal function of cells, have been identified. Results suggest that the HGs serve a storage function in winter and that during the winter period the HG of high RJ producing bees store more proteins than those of Carnica bees.
学科主题	Entomology ; ENTOMOLOGY
语种	英语
出版者	SPRINGER FRANCE
WOS记录号	WOS:000254920800001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5623]
专题	作物科学研究所_重大科学工程项目管理处
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Li, Jianke,Feng, Mao,Zhang, Zhaohui,et al. Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees (Apis mellifera)[J]. APIDOLOGIE,2008,39(2):199-214.
APA	Li, Jianke,Feng, Mao,Zhang, Zhaohui,&Pan, Yinghong.(2008).Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees (Apis mellifera).APIDOLOGIE,39(2),199-214.
MLA	Li, Jianke,et al."Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees (Apis mellifera)".APIDOLOGIE 39.2(2008):199-214.