Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.)

doi:10.1007/s00122-008-0922-0

	Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.)
	Zhou, Lijun 1; Chen, Liangming 1; Jiang, Ling 1; Zhang, Wenwei 1; Liu, Linglong 1; Liu, Xi 1; Zhao, Zhigang 1; Liu, Shijia 1; Zhang, Lujun 1; Wang, Jiankang 2
刊名	THEORETICAL AND APPLIED GENETICS
	2009
卷号	118 期号:3 页码:581-590
ISSN号	0040-5752
DOI	10.1007/s00122-008-0922-0
通讯作者	Zhou, Lijun
英文摘要	Chalkiness of rice grain is an important quality component of rice, as it has a profound influence on eating and milling qualities. We has determined the inheritance of percentage of grain with chalkiness (PGWC) using a set of chromosome segment substitution lines, made from a cross between cv. PA64s and cv. 9311. Two loci controlling PGWC, designated as qPGWC-6 and qPGWC-7, were located on, respectively, chromosomes 6 and 7. Comparisons were made between C-51 (a CSSL harbouring qPGWC-7 and having a chalky endosperm) and the recurrent parent 9311 (translucent endosperm) to characterize the physical and chemical differences between translucent and chalky endosperm. Unlike the translucent endosperm, the chalky endosperm contains loosely packed starch granules, and there were significant difference between C-51 and 9311 for amylopectin structure and degree of crystallinity, but not for either amylose content or starch viscosity. Segregation analysis of the F(2) population from the cross between C-51 and 9311 showed PGWC is a semi-dominant trait, controlled by single nuclear gene. A large F(2) population was constructed from the cross C51 x 9311, and used for the fine mapping of qPGWC-7, which was located to a 44-kb DNA fragment, containing thirteen predicted genes. This result provides a springboard for the map-based cloning of qPGWC-7 and allowed for marker-assisted selection for endosperm texture.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000262577300016
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5542]
专题	作物科学研究所_分子生物学系
作者单位	1.Nanjing Agr Univ, Jiangsu Prov Ctr Plant Gene Engn, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Lijun,Chen, Liangming,Jiang, Ling,et al. Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.)[J]. THEORETICAL AND APPLIED GENETICS,2009,118(3):581-590.
APA	Zhou, Lijun.,Chen, Liangming.,Jiang, Ling.,Zhang, Wenwei.,Liu, Linglong.,...&Wan, Jianmin.(2009).Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.).THEORETICAL AND APPLIED GENETICS,118(3),581-590.
MLA	Zhou, Lijun,et al."Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.)".THEORETICAL AND APPLIED GENETICS 118.3(2009):581-590.