An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis

doi:10.1111/j.1365-313X.2009.03995.x

	An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis
	Gou, Mingyue 1,2,3; Su, Nan 1,2,3; Zheng, Jun 3; Huai, Junling 1,2,3; Wu, Guangheng 4; Zhao, Jinfeng 3; He, Junguang 1,2,3; Tang, Dingzhong 4; Yang, Shuhua 5; Wang, Guoying 1,2,3
刊名	PLANT JOURNAL
	2009
卷号	60 期号:5 页码:757-770
关键词	F-box protein negative regulator disease resistance salicylic acid ASK proteins
ISSN号	0960-7412
DOI	10.1111/j.1365-313X.2009.03995.x
通讯作者	Gou, Mingyue
英文摘要	P>Arabidopsis gain-of-resistance mutants, which show HR-like lesion formation and SAR-like constitutive defense responses, were used well as tools to unravel the plant defense mechanisms. We have identified a novel mutant, designated constitutive expresser of PR genes 30 (cpr30), that exhibited dwarf morphology, constitutive resistance to the bacterial pathogen Pseudomonas syringae and the dramatic induction of defense-response gene expression. The cpr30-conferred growth defect morphology and defense responses are dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), PHYTOALEXIN DEFICIENT 4 (PAD4), and NONRACE-SPECIFIC DISEASE RESISTANCE 1 (NDR1). Further studies demonstrated that salicylic acid (SA) could partially account for the cpr30-conferred constitutive PR1 gene expression, but not for the growth defect, and that the cpr30-conferred defense responses were NPR1 independent. We observed a widespread expression of CPR30 throughout the plant, and a localization of CPR30-GFP fusion protein in the cytoplasm and nucleus. As an F-box protein, CPR30 could interact with multiple Arabidopsis-SKP1-like (ASK) proteins in vivo. Co-localization of CPR30 and ASK1 or ASK2 was observed in Arabidopsis protoplasts. Based on these results, we conclude that CPR30, a novel negative regulator, regulates both SA-dependent and SA-independent defense signaling, most likely through the ubiquitin-proteasome pathway in Arabidopsis.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL PUBLISHING, INC
WOS记录号	WOS:000272188900001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5473]
专题	作物科学研究所_分子生物学系
作者单位	1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China 2.China Agr Univ, Natl Ctr Plant Gene Res Beijing, Beijing 100193, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 5.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Gou, Mingyue,Su, Nan,Zheng, Jun,et al. An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis[J]. PLANT JOURNAL,2009,60(5):757-770.
APA	Gou, Mingyue.,Su, Nan.,Zheng, Jun.,Huai, Junling.,Wu, Guangheng.,...&Wang, Guoying.(2009).An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis.PLANT JOURNAL,60(5),757-770.
MLA	Gou, Mingyue,et al."An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis".PLANT JOURNAL 60.5(2009):757-770.