Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.)

doi:10.1007/s11033-009-9692-2

	Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.)
	He, Junguang 2; Dong, Zhigang 2; Jia, Zhiwei 2; Wang, Jianhua 3; Wang, Guoying 1
刊名	MOLECULAR BIOLOGY REPORTS
	2010
卷号	37 期号:2 页码:865-874
关键词	RdRP SCMV SA Maize
ISSN号	0301-4851
DOI	10.1007/s11033-009-9692-2
通讯作者	He, Junguang
英文摘要	RNA-dependent RNA polymerases (RdRPs) in plants have been reported to be involved in post-transcriptional gene silencing (PTGS) and antiviral defense. In this report, an RdRP gene from maize (ZmRdRP1) was obtained by rapid amplification of cDNA ends (RACE) and RT-PCR. The mRNA of ZmRdRP1 was composed of 3785 nucleotides, including a 167 nt 5' untranslated region (UTR), a 291 nt 3'UTR and a 3327 nt open reading frame (ORF), which encodes a putative protein of 1108 amino acids with an estimated molecular mass of 126.9 kDa and a predicated isoelectric point (pI) of 8.37. Real-time quantitative RT-PCR analysis showed that ZmRdRP1 was elicited by salicylic acid (SA) treatment, methyl jasmonate (MeJA) treatment and sugarcane mosaic virus (SCMV) infection. We silenced ZmRdRP1 by constitutively expressing an inverted-repeat fragment of ZmRdRP1 (ir-RdRP1) in transgenic maize plants. Further studies revealed that the ir-RdRP1 transgenic plants were more susceptible to SCMV infection than wild type plants. Virus-infected transgenic maize plants developed more serious disease symptoms and accumulated more virus than wild type plants. These findings suggested that ZmRdRP1 was involved in antiviral defense in maize.
学科主题	Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000273085800029
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5453]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, State Key Lab Agrobiotecnol, Coll Biol Sci, Beijing 100193, Peoples R China 3.China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	He, Junguang,Dong, Zhigang,Jia, Zhiwei,et al. Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.)[J]. MOLECULAR BIOLOGY REPORTS,2010,37(2):865-874.
APA	He, Junguang,Dong, Zhigang,Jia, Zhiwei,Wang, Jianhua,&Wang, Guoying.(2010).Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.).MOLECULAR BIOLOGY REPORTS,37(2),865-874.
MLA	He, Junguang,et al."Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.)".MOLECULAR BIOLOGY REPORTS 37.2(2010):865-874.