Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity

doi:10.1007/s11033-009-9848-0

	Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity
	Cao, Zuping 2; Jia, Zhiwei 2; Liu, Yongjun 1; Wang, Meng 2; Zhao, Jinfeng 2; Zheng, Jun 1; Wang, Guoying 1
刊名	MOLECULAR BIOLOGY REPORTS
	2010
卷号	37 期号:2 页码:1089-1097
关键词	Cytokinin ZmsHSP Maize CS domain
ISSN号	0301-4851
DOI	10.1007/s11033-009-9848-0
通讯作者	Cao, Zuping
英文摘要	A small HSP gene, ZmsHSP, was isolated from Zea mays. Sequence analysis revealed that the open reading frame of ZmsHSP was 477 bp and that it encodes a protein composed of 159 amino acid residues with a calculated molecular mass of 18.17 kD and a predicated isoelectric point (pI) of 5.63. ZmsHSP contains a CS domain (p23-like domain) and shares similarity with the HSP90 co-chaperone p23. The expression level of ZmsHSP was different among various tissues with the highest expression in leaves and the lowest in silks. Results also showed that the expression of ZmsHSP in maize was significantly up-regulated by dehydration. Transgenic Arabidopsis plants overexpressing ZmsHSP under the control of the CaMV 35S promoter had lower endogenous cytokinin content and showed more sensitivity to cytokinin during the germination and early seedling stage than wild-type plants, suggesting that ZmsHSP might has a function in cytokinin response in Zea mays.
学科主题	Biochemistry & Molecular Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000273085800056
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5452]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Cao, Zuping,Jia, Zhiwei,Liu, Yongjun,et al. Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity[J]. MOLECULAR BIOLOGY REPORTS,2010,37(2):1089-1097.
APA	Cao, Zuping.,Jia, Zhiwei.,Liu, Yongjun.,Wang, Meng.,Zhao, Jinfeng.,...&Wang, Guoying.(2010).Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity.MOLECULAR BIOLOGY REPORTS,37(2),1089-1097.
MLA	Cao, Zuping,et al."Constitutive expression of ZmsHSP in Arabidopsis enhances their cytokinin sensitivity".MOLECULAR BIOLOGY REPORTS 37.2(2010):1089-1097.