Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure

doi:10.1111/tpj.12086

	Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure
	Li, Baochun 1; Choulet, Frederic 2,3; Heng, Yanfang 1,4; Hao, Weiwei 1; Paux, Etienne 2,3; Liu, Zhao 1; Yue, Wei 1; Jin, Weiwei 4; Feuillet, Catherine 2,3; Zhang, Xueyong 1
刊名	PLANT JOURNAL
	2013
卷号	73 期号:6 页码:952-965
关键词	Triticum aestivum Chromosome 3B centromere retrotransposon CENH3 evolution
ISSN号	0960-7412
DOI	10.1111/tpj.12086
通讯作者	Li, Baochun
英文摘要	The physical map of the hexaploid wheat chromosome3B was screened using centromeric DNA probes. A 1.1-Mb region showing the highest number of positive bacterial artificial chromosome (BAC) clones was fully sequenced and annotated, revealing that 96% of the DNA consisted of transposable elements, mainly long terminal repeat (LTR) retrotransposons (88%). Estimation of the insertion time of the transposable elements revealed that CRW (also called Cereba) and Quinta are the youngest elements at the centromeres of common wheat (Triticumspp.) and its diploid ancestors, with Quinta being younger than CRW in both diploid and hexaploid wheats. Chromatin immunoprecipitation experiments revealed that both CRW and Quinta families are targeted by the centromere-specific histone H3 variant CENH3. Immuno colocalization of retroelements and CENH3 antibody indicated that a higher proportion of Quinta than CRWs was associated with CENH3, although CRWs were more abundant. Long arrays of satellite repeats were also identified in the wheat centromere regions, but they lost the ability to bind with CENH3. In addition to transposons, two functional genes and one pseudogene were identified. The gene density in the centromere appeared to be between three and four times lower than the average gene density of chromosome3B. Comparisons with related grasses also indicated a loss of microcollinearity in this region. Finally, comparison of centromeric sequences of Aegilops tauschii (DD), Triticum boeoticum (AA) and hexaploid wheat revealed that the centromeres in both the polyploids and diploids are still undergoing dynamic changes, and that the new CRWs and Quintas may have undertaken the core role in kinetochore formation.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000316217600007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5065]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Crop Gene Resource & Germplasm Enhancemen, Beijing 100081, Peoples R China 2.INRA, Genet Divers & Ecophysiol Cereals UMR1095, F-63100 Clermont Ferrand, France 3.Univ Clermont Ferrand, Genet Divers & Ecophysiol Cereals UMR1095, F-63100 Clermont Ferrand, France 4.China Agr Univ, Natl Maize Improvement Ctr, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, Baochun,Choulet, Frederic,Heng, Yanfang,et al. Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure[J]. PLANT JOURNAL,2013,73(6):952-965.
APA	Li, Baochun.,Choulet, Frederic.,Heng, Yanfang.,Hao, Weiwei.,Paux, Etienne.,...&Zhang, Xueyong.(2013).Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure.PLANT JOURNAL,73(6),952-965.
MLA	Li, Baochun,et al."Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure".PLANT JOURNAL 73.6(2013):952-965.