Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize

doi:10.1007/s11240-013-0348-5

	Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize
	Zhang, Yuwen 1,2; Liu, Yunjun 2; Ren, Yuan 1; Liu, Yan 2; Liang, Gemei 3; Song, Fuping 3; Bai, Shuxiong 3; Wang, Jianhua 1; Wang, Guoying 2
刊名	PLANT CELL TISSUE AND ORGAN CULTURE
	2013
卷号	115 期号:2 页码:151-158
关键词	Cry1Ie Transgenic maize Cry1Ac-resistant Cotton bollworm
ISSN号	0167-6857
DOI	10.1007/s11240-013-0348-5
通讯作者	Zhang, Yuwen
英文摘要	Although transgenic crops expressing either Cry1Ab or Cry1Ac, both derived from Bacillus thuringiensis (Bt), have been used commercially, the evolution of insects resistance to these CRY proteins has become a challenge. Thus, it has been proposed that co-expression of two Bt proteins with different modes of action may delay the development of resistance to Bt. However, few Bt proteins have been identified as having different modes of action from those of Cry1Ab or Cry1Ac. In this study, transgenic lines of maize over-expressing either Cry1Ie or Cry1Ac gene have been developed. Several independent transgenic lines with one copy of the foreign gene were identified by Southern blot analysis. Bioassays in the laboratory showed that the transgenic plants over-expressing Cry1Ie were highly toxic against the wild-type cotton bollworm (Heliothis armigera), producing mortality levels of 50 % after 6 days of exposure. However, the mortality caused by these plants was lower than that caused by the Cry1Ac transgenic plants (80 %) and MON810 plants expressing Cry1Ab (100 %), which both exhibited low toxicity toward the Cry1Ac-resistant cotton bollworm. In contrast, three transgenic maize lines expressing Cry1Ie induced higher mortality against this pest and were also highly toxic to the Asian corn borer (Ostrinia furnacalis) in the field. These results indicate that the Cry1Ie protein has a different mode of action than the Cry1Ab and Cry1Ac proteins. Therefore, the use of transgenic plants expressing Cry1Ie might delay the development of Bt-resistant insects in the field.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000325849300004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4990]
专题	作物科学研究所_分子生物学系
作者单位	1.China Agr Univ, Coll Agr & Biotechnol, Beijing 100094, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 3.CAAS, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yuwen,Liu, Yunjun,Ren, Yuan,et al. Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2013,115(2):151-158.
APA	Zhang, Yuwen.,Liu, Yunjun.,Ren, Yuan.,Liu, Yan.,Liang, Gemei.,...&Wang, Guoying.(2013).Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize.PLANT CELL TISSUE AND ORGAN CULTURE,115(2),151-158.
MLA	Zhang, Yuwen,et al."Overexpression of a novel Cry1Ie gene confers resistance to Cry1Ac-resistant cotton bollworm in transgenic lines of maize".PLANT CELL TISSUE AND ORGAN CULTURE 115.2(2013):151-158.