A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat

doi:10.1111/tpj.12449

	A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat
	He, Xue 1; Fang, Jingjing 1,2; Li, Jingjuan 1,3; Qu, Baoyuan 1; Ren, Yongzhe 1,4; Ma, Wenying 1; Zhao, Xueqiang 1; Li, Bin 1; Wang, Daowen 1; Li, Zhensheng 1
刊名	PLANT JOURNAL
	2014
卷号	77 期号:6 页码:931-943
关键词	primary root length Triticum aestivum L. reactive oxygen species root meristem size brassinosteroid proteomics peroxidase TGF-beta receptor-interacting protein-1
ISSN号	0960-7412
DOI	10.1111/tpj.12449
通讯作者	He, Xue
英文摘要	Previously we identified a major quantitative trait locus (QTL) qTaLRO-B1 for primary root length (PRL) in wheat. Here we compare proteomics in the roots of the qTaLRO-B1 QTL isolines 178A, with short PRL and small meristem size, and 178B, with long PRL and large meristem size. A total of 16 differentially expressed proteins were identified: one, transforming growth factor (TGF)-beta receptor-interacting protein-1 (TaTRIP1), was enriched in 178A, while various peroxidases (PODs) were more abundantly expressed in 178B. The 178A roots showed higher TaTRIP1 expression and lower levels of the unphosphorylated form of the brassinosteroid (BR) signaling component BZR1, lower expression of POD genes and reduced POD activity and accumulation of the superoxide anion O-2(-) in the root elongation zone compared with the 178B roots. Low levels of 24-epibrassinolide increased POD gene expression and root meristem size, and rescued the short PRL phenotype of 178A. TaTRIP1 directly interacted with the BR receptor TaBRI1 of wheat. Moreover, overexpressing TaTRIP1 in Arabidopsis reduced the abundance of unphosphorylated BZR1 protein, altered the expression of BR-responsive genes, inhibited POD activity and accumulation of the O-2(-) in the root tip and inhibited root meristem size. Our data suggested that TaTRIP1 is involved in BR signaling and inhibited root meristem size, possibly by reducing POD activity and accumulation of O-2(-) in the root tip. We further demonstrated a negative correlation between the level of TaTRIP1 mRNA and PRL of landraces and modern wheat varieties, providing a valuable insight for better understanding of the molecular mechanism underlying the genotypic differences in root morphology of wheat in the future.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000332503500009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4924]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Sci, Beijing 100101, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 10081, Peoples R China 3.Shandong Acad Agr Sci, Shandong Key Lab Greenhouse Vegetable Biol, Inst Vegetables & Flowers, Jinan 250100, Peoples R China 4.Shangqiu Normal Univ, Coll Life Sci, Shangqiu 476000, Peoples R China
推荐引用方式 GB/T 7714	He, Xue,Fang, Jingjing,Li, Jingjuan,et al. A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat[J]. PLANT JOURNAL,2014,77(6):931-943.
APA	He, Xue.,Fang, Jingjing.,Li, Jingjuan.,Qu, Baoyuan.,Ren, Yongzhe.,...&Tong, Yiping.(2014).A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat.PLANT JOURNAL,77(6),931-943.
MLA	He, Xue,et al."A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat".PLANT JOURNAL 77.6(2014):931-943.