DWARF3 Participates in an SCF Complex and Associates with DWARF14 to Suppress Rice Shoot Branching

doi:10.1093/pcp/pcu045

	DWARF3 Participates in an SCF Complex and Associates with DWARF14 to Suppress Rice Shoot Branching
	Zhao, Jinfeng 1; Wang, Tao 1; Wang, Minxia 1; Liu, Yuanyuan 1; Yuan, Shoujiang 2; Gao, Yanan 3; Yin, Liang 2; Sun, Wei 2; Peng, Lixiang 1; Zhang, Wenhui 3
刊名	PLANT AND CELL PHYSIOLOGY
	2014
卷号	55 期号:6 页码:1096-1109
关键词	GR24 High-tillering dwarf SCF complex Shoot branching Strigolactones
ISSN号	0032-0781
DOI	10.1093/pcp/pcu045
通讯作者	Zhao, Jinfeng
英文摘要	Strigolactones (SLs) are a novel class of plant hormones that inhibit shoot branching. Currently, two proteins in rice are thought to play crucial roles in SL signal transduction. DWARF14 (D14), an alpha/beta hydrolase, is responsible for SL perception, while DWARF3 (D3), an F-box protein with leucine-rich repeats, is essential for SL signal transduction. However, how these two proteins transmit SL signals to downstream factors remains unclear. Here, we characterized a high-tillering dwarf rice mutant, gsor300097, which is insensitive to GR24, a synthetic analog of SL. Mapping and sequencing analysis showed that gsor300097 is a novel allelic mutant of D3, in which a nonsense mutation truncates the protein from 720 to 527 amino acids. The D3 gene was strongly expressed in root, leaf, shoot base and panicle. Nuclear-localized F-box protein D3 played a role in the SCF complex by interacting with OSK1, OSK5 or OSK20 and OsCullin1. In addition, D3 associated with D14 in a GR24-dependent manner in vivo. Taken together, our findings suggested that D3 assembled into an SCFD3 complex and associated with D14 to suppress rice shoot branching.
学科主题	Plant Sciences ; Cell Biology ; CELL BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000338127800006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4879]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Shandong Rice Res Inst, Jinan 250100, Peoples R China 3.Liaocheng Univ, Sch Life Sci, Liaocheng 252059, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jinfeng,Wang, Tao,Wang, Minxia,et al. DWARF3 Participates in an SCF Complex and Associates with DWARF14 to Suppress Rice Shoot Branching[J]. PLANT AND CELL PHYSIOLOGY,2014,55(6):1096-1109.
APA	Zhao, Jinfeng.,Wang, Tao.,Wang, Minxia.,Liu, Yuanyuan.,Yuan, Shoujiang.,...&Li, Xueyong.(2014).DWARF3 Participates in an SCF Complex and Associates with DWARF14 to Suppress Rice Shoot Branching.PLANT AND CELL PHYSIOLOGY,55(6),1096-1109.
MLA	Zhao, Jinfeng,et al."DWARF3 Participates in an SCF Complex and Associates with DWARF14 to Suppress Rice Shoot Branching".PLANT AND CELL PHYSIOLOGY 55.6(2014):1096-1109.