Fine mapping of a minor-effect QTL, DTH12, controlling heading date in rice by up-regulation of florigen genes under long-day conditions

doi:10.1007/s11032-014-0035-1

	Fine mapping of a minor-effect QTL, DTH12, controlling heading date in rice by up-regulation of florigen genes under long-day conditions
	Zhong, Zhengzheng 1,2; Wu, Weixun 2; Wang, Hongjun 2; Chen, Liping 2; Liu, Linglong 2; Wang, Chunming 2; Zhao, Zhigang 2; Lu, Guangwen 2; Gao, He 2; Wei, Xiangjin 2
刊名	MOLECULAR BREEDING
	2014
卷号	34 期号:2 页码:311-322
关键词	Oryza sativa DTH12 Heading date Minor-effect QTL Near-isogenic line
ISSN号	1380-3743
DOI	10.1007/s11032-014-0035-1
通讯作者	Zhong, Zhengzheng
英文摘要	Heading date is a key determinant of regional and seasonal adaptation in rice (Oryza sativa L.). Aminor-effect quantitative trait locus (QTL), QTL for Days to heading 12a (qDTH-12a), with unknown genetic action was previously coarsely detected in a recombinant inbred line population. The study reported here was designed to better define the qDTH-12a locus (designated as DTH12) in advanced segregating populations. DTH12 was initially verified in chromosome segment substitution line CSSL84. A CSSL84/Asominori//Asominori BC4F2 population was then developed, and a near-isogenic line (NIL), NIL(DTH12), was subsequently selected from this population using marker-assisted tracking that headed 8 days later than Asominori under long-day (LD) conditions but which was not significantly different in heading date in short-day environments. Using 358 Asominori/NIL(DTH12) F-2:3 families grown underLD conditions, we were able to initially map DTH12 to a 26-cM interval between markers InDel12-1 and RM6296. F-3 individuals heterozygous for the DTH12 regions were then chosen, and 2,388 F-4:5 families were used for fine mapping. DTH12 was finally dissected as a single gene and delimited to a 153-kb genomic region with 32 open reading frames. Compared with Asominori, NIL(DTH12) showed reduced transcription of the florigen genes Heading date 3a and RICE FLOWERING LOCUS T 1, suggesting that DTH12 functions as an up-regulator of florigen genes during floral induction under LD conditions. DTH12 was also found to have an important role in rice adaptation and breeding for precise control of seed maturity. These findings provide a firm basis for cloning this minor-effect QTL involved in rice flowering.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000342164700004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4854]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Zhengzheng,Wu, Weixun,Wang, Hongjun,et al. Fine mapping of a minor-effect QTL, DTH12, controlling heading date in rice by up-regulation of florigen genes under long-day conditions[J]. MOLECULAR BREEDING,2014,34(2):311-322.
APA	Zhong, Zhengzheng.,Wu, Weixun.,Wang, Hongjun.,Chen, Liping.,Liu, Linglong.,...&Wan, Jianmin.(2014).Fine mapping of a minor-effect QTL, DTH12, controlling heading date in rice by up-regulation of florigen genes under long-day conditions.MOLECULAR BREEDING,34(2),311-322.
MLA	Zhong, Zhengzheng,et al."Fine mapping of a minor-effect QTL, DTH12, controlling heading date in rice by up-regulation of florigen genes under long-day conditions".MOLECULAR BREEDING 34.2(2014):311-322.