A high density GBS map of bread wheat and its application for dissecting complex disease resistance traits

doi:10.1186/s12864-015-1424-5

	A high density GBS map of bread wheat and its application for dissecting complex disease resistance traits
	Li, Huihui 1,2; Vikram, Prashant 1; Prakash Singh, Ravi 1; Kilian, Andrzej 3; Carling, Jason 3; Song, Jie 3; Andres Burgueno-Ferreira, Juan 1; Bhavani, Sridhar 1; Huerta-Espino, Julio 1; Payne, Thomas 1
刊名	BMC GENOMICS
	2015
卷号	16 页码:-
关键词	Consensus map Genotyping-by-sequencing (GBS) QTL mapping Rust resistance Segregation distortion Wheat
ISSN号	1471-2164
DOI	10.1186/s12864-015-1424-5
通讯作者	Li, Huihui
英文摘要	Background: Genotyping-by-sequencing (GBS) is a high-throughput genotyping approach that is starting to be used in several crop species, including bread wheat. Anchoring GBS tags on chromosomes is an important step towards utilizing them for wheat genetic improvement. Here we use genetic linkage mapping to construct a consensus map containing 28644 GBS markers.Results: Three RIL populations, PBW343 x Kingbird, PBW343 x Kenya Swara and PBW343 x Muu, which share a common parent, were used to minimize the impact of potential structural genomic variation on consensus-map quality. The consensus map comprised 3757 unique positions, and the average marker distance was 0.88 cM, obtained by calculating the average distance between two adjacent unique positions. Significant variation of segregation distortion was observed across the three populations. The consensus map was validated by comparing positions of known rust resistance genes, and comparing them to wheat reference genome sequences recently published by the International Wheat Genome Sequencing Consortium, Rye and Ae. tauschii genomes. Three well-characterized rust resistance genes (Sr58/Lr46/Yr29, Sr2/Yr30/Lr27, and Sr57/Lr34/Yr18) and 15 published QTLs for wheat rusts were validated with high resolution. Fifty-two per cent of GBS tags on the consensus map were successfully aligned through BLAST to the right chromosomes on the wheat reference genome sequence.Conclusion: The consensus map should provide a useful basis for analyzing genome-wide variation of complex traits. The identified genes can then be explored as genetic markers to be used in genomic applications in wheat breeding.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000351894700001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4742]
专题	作物科学研究所_分子生物学系
作者单位	1.Int Maize & Wheat Improvement Ctr CIMMYT, Mexico City 06600, DF, Mexico 2.Chinese Acad Agr Sci, Inst Crop Sci, CIMMYT China Off, Beijing 100081, Peoples R China 3.DArT, Divers Array Technol, Canberra, ACT, Australia
推荐引用方式 GB/T 7714	Li, Huihui,Vikram, Prashant,Prakash Singh, Ravi,et al. A high density GBS map of bread wheat and its application for dissecting complex disease resistance traits[J]. BMC GENOMICS,2015,16:-.
APA	Li, Huihui.,Vikram, Prashant.,Prakash Singh, Ravi.,Kilian, Andrzej.,Carling, Jason.,...&Singh, Sukhwinder.(2015).A high density GBS map of bread wheat and its application for dissecting complex disease resistance traits.BMC GENOMICS,16,-.
MLA	Li, Huihui,et al."A high density GBS map of bread wheat and its application for dissecting complex disease resistance traits".BMC GENOMICS 16(2015):-.