The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2

doi:10.1016/j.molp.2015.03.005

	The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2
	Wang, Qin 1,2,6; Barshop, William D.3; Bian, Mingdi 4; Vashisht, Ajay A.3; He, Reqing 2; Yu, Xuhong 6; Liu, Bin 5; Nguyen, Paula 6; Liu, Xuanming 2; Zhao, Xiaoying 2
刊名	MOLECULAR PLANT
	2015
卷号	8 期号:4 页码:631-643
关键词	light regulation light signaling physiology of plant growth Arabidopsis
ISSN号	1674-2052
DOI	10.1016/j.molp.2015.03.005
通讯作者	Wang, Qin
英文摘要	Arabidopsis cryptochrome 2 (CRY2) is a blue light receptor that mediates light inhibition of hypocotyl elongation and long-day promotion of floral initiation. CRY2 is known to undergo blue light-dependent phosphorylation, which is believed to serve regulatory roles in the function of CRY2. We report here on a biochemical and genetics study of CRY2 phosphorylation. Using mass spectrometry analysis, we identified three serine residues in the CCE domain of CRY2 (S588, S599, and S605) that undergo blue light-dependent phosphorylation in Arabidopsis seedlings. A study of serine-substitution mutations in the CCE domain of CRY2 demonstrates that CRY2 contains two types of phosphorylation in the CCE domain, one in the serine cluster that causes electrophoretic mobility upshift and the other outside the serine cluster that does not seem to cause mobility upshift. We showed that mutations in the serine residues within and outside the serine cluster diminished blue light-dependent CRY2 phosphorylation, degradation, and physiological activities. These results support the hypothesis that blue light-dependent phosphorylation of the CCE domain determines the photosensitivity of Arabidopsis CRY2.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000352306500011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4729]
专题	作物科学研究所_分子生物学系
作者单位	1.Fujian Agr & Forestry Univ, Basic Forestry & Prote Res Ctr, Fuzhou, Peoples R China 2.Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China 3.Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90095 USA 4.Jilin Univ, Lab Soil & Plant Mol Genet, Coll Plant Sci, Changchun 130062, Peoples R China 5.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 6.Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA
推荐引用方式 GB/T 7714	Wang, Qin,Barshop, William D.,Bian, Mingdi,et al. The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2[J]. MOLECULAR PLANT,2015,8(4):631-643.
APA	Wang, Qin.,Barshop, William D..,Bian, Mingdi.,Vashisht, Ajay A..,He, Reqing.,...&Lin, Chentao.(2015).The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2.MOLECULAR PLANT,8(4),631-643.
MLA	Wang, Qin,et al."The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2".MOLECULAR PLANT 8.4(2015):631-643.